Investigating the interactomic landscape of survival motor neuron (SMN) and the SMNΔ7 truncated protein [0.03%]
Survival Motor Neuron (SMN)及其截短蛋白SMNΔ7的互作蛋白质组景观研究
Bobby Beaumont,Silvia A Synowsky,Sally L Shirran et al.
Bobby Beaumont et al.
This protocol describes a methodology combining TurboID - a recently developed proximity biotinylation technique - with conventional epitope-tag based co-immunoprecipitation (Co-IP) to analyze protein-protein interactions (PPIs) in cell cul...
Antigen retrieval-immunofluorescence on free floating sections to visualize the liver lobule and its cellular makeup [0.03%]
抗原检索-免疫荧光（游离漂浮切片法）可视化肝小叶及其细胞组成
Hawon Yoon,Aashita Singh Rajput,Kanesha Lenay Travis et al.
Hawon Yoon et al.
Method summary: Paraformaldehyde-fixed mouse liver samples are sectioned on a vibratome at 100 µm. The free-floating sections are permeabilized, followed by exposure to one of two heat-induced antigen retrieval methods s...
Special approach of droplet digital polymerase chain reaction (ddPCR) for transgene stability of a Chinese hamster ovary (CHO) cell line [0.03%]
一种用于中国仓鼠卵巢(CHO)细胞系中外源基因稳定性的 Droplet Digital Polymerase Chain Reaction(ddPCR) 特异方法学研究
Shu-Min Zhang,Michele Shannon,Wendy Halsey et al.
Shu-Min Zhang et al.
Cell line stability of mammalian cell lines is an important concern for manufacturing of biopharmaceuticals. Characterization of the master cell bank (MCB) for monoclonal antibody A (Mab A) via multiplex fluorescence in situ hybridization (...
Strand-specific quantification of L1 ORF0 and related transcripts by multiplex reverse transcription with tagged primers [0.03%]
带有标记引物的多重逆转录定量L1 ORF0及相关转录物的链特异性定量方法
Kazunari Mizuno,Yutaka Nakachi,Emi Kiyota et al.
Kazunari Mizuno et al.
Long interspersed nuclear element-1 (LINE-1 or L1) is the only autonomously active retrotransposon in the human genome and produces both sense and antisense transcripts from its 5' untranslated region (5'UTR) of L1Hs, a human-specific L1 su...
Mustafa Ahmad Munawar,Sofie Mohlin
Mustafa Ahmad Munawar
Quantitative and unbiased lung alveolar septum assessment in an LPS experimental mouse model using 2D-spatial correlation image analysis from hematoxylin and eosin slides [0.03%]
基于血红蛋白和伊红载玻片的二维空间相关图像分析，对LPS实验小鼠模型进行定量和无偏肺泡间隔评估
Micaela Lopassio,María José García,Leonel Malacrida
Micaela Lopassio
Introduction: Quantitative assessment of lung tissue architecture is essential for evaluating disease progression in experimental models of acute lung injury (ALI). However, conventional methods for measuring alveolar septum thickness rely ...
In vivo RNA targeting in the nematode Caenorhabditis elegans using exogenous catalytic DNA [0.03%]
利用外源催化性DNA在秀丽隐杆线虫中实现体内RNA特异性降解技术
Eriс Khananshvilli,Ayelet-Chen Abraham,Netanel Cohen et al.
Eriс Khananshvilli et al.
Catalytic DNA molecules (DNAzymes) offer a programmable means to manipulate RNA without genomic modification. Although DNAzymes have been thoroughly characterized in culture and in various animals, their utility in the prevalent model syste...
Cecilie Mondrup Jacobsen,Luisa Matos do Canto,Estrid Høgdall et al.
Cecilie Mondrup Jacobsen et al.
Comparative performance of high-throughput qPCR, qPCR, and digital PCR for antibiotic resistance gene monitoring in tropical water systems [0.03%]
高通量qPCR、qPCR和数字PCR在热带水体抗生素抗性基因监测中的性能比较
Thitima Srathongneam,Phongsawat Paisantham,Andrew C Singer et al.
Thitima Srathongneam et al.
Antibiotic resistance genes (ARGs) are important contaminants in water systems, and their detection depends strongly on methodological sensitivity. This study compared three molecular platforms, high-throughput quantitative polymerase chain...
Comparative Study
BioTechniques. 2026 Jan-Dec;78(1-12):158-169. DOI:10.1080/07366205.2026.2649861 2026
A simple and rapid agarose gel electrophoresis method to assess CpG methylation of DNA [0.03%]
一种简单快速的用于评估DNA甲基化情况的琼脂糖凝胶电泳方法
Marco Paliza-Carre,Ge Chen,Shahrokh Shabahang
Marco Paliza-Carre
The presence of 5-methylcytosine at CpG sites in mammalian DNA plays a significant role in various biological processes, both normal and aberrant. Similarly, CpG site methylation status of therapeutic DNAs can affect activity and therapeuti...