Qichang Nie,Terrence Chi-Kong Lau
Qichang Nie
Targeted protein degradation is an emerging concept of drug discovery to selectively eliminate the pathogenic proteins by activating their degradation in cells. The proteolysis-targeting chimeras (PROTACs) are bifunctional small molecules t...
Assessment of Bacterial N-Degron Proteolysis by a Dual-Fluorescence Reporter [0.03%]
利用双荧光报告子评估细菌N-末端降解蛋白酶体的功能
Pratistha Kandel,Patrick C Beardslee,Karl R Schmitz
Pratistha Kandel
Many organisms possess N-degron pathways that link the proteolytic stability of individual proteins to the identity of their N-terminal amino acid. The set of N-degrons recognized by proteolytic machinery, and the regulatory paradigms leadi...
Evaluation of Degron Motifs in Escherichia coli Using a Fluorescent Reporter [0.03%]
利用荧光报告蛋白评估大肠杆菌中的Degron motifs功能
Matylda A Izert-Nowakowska,Patrycja E Szybowska,Maria M Klimecka et al.
Matylda A Izert-Nowakowska et al.
Fluorescent reporters provide a useful tool for studying degron motifs. Fusing a degron of interest to a fluorescent protein allows to accurately track protein levels overtime to characterize the degradation kinetics of studied degrons. Her...
Production of Purified Proteins Tagged with ssrA-Derived Degrons and Their Degradation by the Escherichia coli ClpXP System [0.03%]
带有ssrA衍生degron的纯化蛋白的制备及其通过大肠杆菌ClpXP系统降解
Maria M Klimecka,Matylda A Izert-Nowakowska,Maria W Górna
Maria M Klimecka
The ssrA degron is widely employed in fusion proteins to regulate protein stability in bacteria or to serve as an interaction module. These uses take advantage of the ssrA tag's dual functionality-its ability to bind the SspB adaptor and to...
Degrade Green Fluorescent Protein (deGradFP) Method in Trypanosoma brucei [0.03%]
布鲁氏锥虫中的绿色荧光蛋白（deGradFP）降解方法
Midori Ishii,Bungo Akiyoshi
Midori Ishii
Degrade green fluorescent protein (deGradFP) is a degron method that was originally developed in Drosophila melanogaster. It targets GFP-fusion proteins to an endogenous ubiquitin-proteasome pathway using an anti-GFP nanobody. We have adapt...
Alexandra Klein,Alice McDowell,Christian R S Reis et al.
Alexandra Klein et al.
The ability to specifically degrade one protein in a cell provides an immediate insight into any resulting phenotype and thus function that may be occluded by secondary effects using other techniques. The auxin-inducible degron is a method ...
Protein Depletion in Caenorhabditis elegans Using the Auxin-Inducible Degradation System [0.03%]
利用植物诱导降解系统在秀丽隐杆线虫中进行蛋白质耗竭
Jordan D Ward
Jordan D Ward
The auxin-inducible degradation (AID) system is a powerful tool in modern molecular genetics that allows for conditional protein depletion. In Caenorhabditis elegans, tools exist to allow for rapid, tissue-specific depletion of target prote...
Ulrike Vavra,Christiane Veit,Richard Strasser
Ulrike Vavra
The endoplasmic reticulum (ER) is the site where proteins that are synthesized and destined for the secretory pathway fold into their native conformations. Genetic mutations, oxidative stress, reduced glycosylation, and disruption to ER fol...
Catherine L Ingram,Radosław P Nowak,Thomas M Geiger
Catherine L Ingram
Transcription factors are an exciting class of drug targets. Recent developments in small molecule-induced proximity allow for small molecule control of their activity by recruitment of epigenetic proteins. In this chapter, we describe the ...
Dynamic Control of Cell Signaling with Optogenetic Modulation of Protein Abundance in Living Mammalian Cells [0.03%]
利用光遗传学调节活体哺乳动物细胞中蛋白质丰度动态控制细胞信号转导
Yu-En Huang,Payel Mondal,Huaxun Fan et al.
Yu-En Huang et al.
Precise modulation of protein levels is essential for investigating the dynamics of signaling pathways. Degradation-based approaches allow rapid reduction of protein abundance, and their combination with optogenetic regulation enables contr...