Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy [0.03%]
免疫荧光显微镜在细菌表面合成粘附素中的追踪作用
Sofía Fraile,Jaime Fernández de Córdoba,Víctor de Lorenzo et al.
Sofía Fraile et al.
Programmable bacterial adhesion enables applications in biocatalysis, living materials, and targeted drug delivery. This functionality can be achieved through the expression of synthetic adhesins in bacterial cells. These molecules are hybr...
Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits [0.03%]
mRNA显示筛选的分析方法及命中化合物的优化
Pearl Qi,Colin M Leaf,Richard W Roberts et al.
Pearl Qi et al.
mRNA display enables the selection of peptide and protein ligands from libraries containing more than a trillion unique sequences. Identifying optimal target binders, however, requires extensive post-selection analysis and optimization, whi...
High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification [0.03%]
高效计算在串联质谱肽段识别中的应用
Chuang Li,Qiang He,Tao Chen et al.
Chuang Li et al.
Tandem mass spectrometry (MS/MS) has become a cornerstone technology in large-scale proteomics, producing vast datasets that present considerable computational challenges for peptide identification. Conventional database-search-based approa...
Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality [0.03%]
工程和适应含二硫键的蛋白质以实现细胞内功能
Elise Sylvander,Michael W Traxlmayr
Elise Sylvander
Disulfide bonds are key structural components ensuring proper protein folding. Those bonds, however, do not form in the reducing intracellular environment, often resulting in misfolded, unstable proteins with subpar function. To enable intr...
Guodong Min,Huan Peng
Guodong Min
Proteins are central to nearly all biological processes, mediating enzymatic catalysis, structural scaffolding, and molecular signaling. Deciphering how linear amino acid sequences encode three-dimensional conformations and biological funct...
Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display [0.03%]
基于mRNA显示的体外蛋白质和肽文库筛选方法
Colin M Leaf,Pearl Qi,Richard W Roberts et al.
Colin M Leaf et al.
mRNA display is a powerful in vitro selection technique that enables the discovery of peptide and protein ligands from libraries exceeding 10 trillion unique sequences. The technique allows for exquisite control over binding stringency and ...
Sota Uchida,Hiroaki Suga,Yuki Goto
Sota Uchida
Prenylated peptides, endowed with hydrophobic prenyl groups, are widely found as secondary metabolites in nature. To develop de novo artificial prenylated peptide ligands with selective affinity for a target protein of choice, a random pren...
Phage Display Biopanning Strategy for Discovery of Nanoparticle-Binding Peptides [0.03%]
用于发现结合纳米颗粒的肽类的噬菌体展示生物筛选策略
Piotr Golec,Grzegorz Węgrzyn
Piotr Golec
The phage display technology allows to select peptides, exposed on the surface of bacteriophage virions, which can specifically bind to virtually any materials, including proteins, lipids, carbohydrates, and other organic macromolecules, as...
Belle Li Ling,Angela Chiew Wen Ch&#x;ng,Yee Siew Choong et al.
Belle Li Ling et al.
Antibodies, central components of the adaptive immune system, possess the remarkable ability to specifically recognize and bind to foreign substances known as antigens. This recognition occurs through the interaction of the antibody's antig...
Yeast Molecular Display for the Screening and Production of Immunological Proteins Using Nanobody as an Example [0.03%]
以纳米抗体为例的酵母分子展示筛选及免疫学蛋白表达研究
Seiji Shibasaki,Wataru Aoki,Mitsuyoshi Ueda
Seiji Shibasaki
Yeast molecular display is a convenient tool for developing and improving recombinant proteins and assessing their functions. Molecular display using Saccharomyces cerevisiae has been applied for screening valuable biochemical molecules, in...