YeastSAM: A Deep Learning Model for Accurate Segmentation of Yeast Cells [0.03%]
酵母细胞精确分割的深度学习模型Yeastsam
Yonghao Zhao,Zhouyuan Zhu,Sen Yang et al.
Yonghao Zhao et al.
Cell segmentation-the process of defining cell outlines in microscopy images-is essential for quantitative image analysis. Segmentation of budding yeast is challenging due to their asymmetric cell division and mother-bud morphology. Consequ...
Endothelial β-PIX (ARHGEF7) supports actomyosin mediated expulsion of VWF through dynamic reorganization of the cytoskeleton [0.03%]
内皮细胞β-PIX（ARHGEF7）通过细胞骨架的动态重排支持肌动蛋白肌球蛋白介导的VWF排出
Sammy El-Mansi,Karishma M Deegan,Thomas D Nightingale
Sammy El-Mansi
Endothelial cells promote thrombosis and haemostasis through the secretion of von Willebrand Factor (VWF) from their secretory granules - the Weibel-Palade bodies (WPBs). In response to specific stimuli, dynamic actin nucleation and remodel...
Sequence and structure of protein binding sites in RNA impact biomolecular condensates [0.03%]
RNA蛋白质结合位点的序列和结构影响生物分子凝聚体
Sierra J Cole,Scott R Allen,Bryan B Guzmán et al.
Sierra J Cole et al.
Biomolecular condensates are central to subcellular compartmentalization and RNA regulation. In the multinucleate fungus Ashbya gossypii, condensates composed of Whi3 protein and CLN3 mRNA help ensure nuclear cycle asynchrony in a shared cy...
Tubulin Acetylation Deficiency Promotes Axonemal Turnover and Increases Cytoplasmic Microtubules [0.03%]
微管乙酰化缺乏促进轴丝周转并增加细胞质微管数量
Tomohiro Kubo,Natsumi Tajima-Shirasaki,Rinka Sasaki et al.
Tomohiro Kubo et al.
Tubulin post-translational modifications regulate microtubule dynamics. Although α-tubulin acetylation has been linked to microtubule stability, how this modification affects the overall organization of cellular microtubules remains obscur...
A MAP kinase cascade modulates expression of late G1 phase cyclins in budding yeast [0.03%]
芽裂酵母中一种MAP激酶级联调节晚G1期周期蛋白的表达
Navid Zebarjadi,Douglas R Kellogg
Navid Zebarjadi
Expression of late G1 phase cyclins is the critical molecular event that marks commitment to enter the cell cycle. In budding yeast, late G1 phase cyclins initiate and sustain growth of a new daughter bud, so their expression also marks the...
Septins associate with AP-3 to support trafficking to the vacuole/lysosome in yeast [0.03%]
Septin与AP-3相互作用以支持酵母细胞囊泡运输到液泡/溶酶体中
Mitchell Leih,Michaela McCright,Cortney Angers et al.
Mitchell Leih et al.
Adaptor protein complex 3 (AP-3) mediates clathrin-independent transport to lysosomes, yet accessory factors supporting this pathway remain incompletely defined. In Saccharomyces cerevisiae, the C-terminal intrinsically disordered regions (...
Polarized anionic phospholipids and exocytosis are implicated in the polarized recruitment of budding yeast AP180, an endocytic initiator [0.03%]
极化阴离子磷脂和胞吐作用与芽殖酵母AP180(一种胞吞起始因子)的极性募集有关
Paul Marchando,Gean Hu,Feng Yuan et al.
Paul Marchando et al.
Understanding of the mechanisms that initiate clathrin-mediated endocytosis (CME) is incomplete. Recent studies in budding yeast identified the endocytic adaptor proteins Yap1801/Yap1802 (budding yeast AP180) as key CME factors that promote...
Cytokinetic abscission failures in a polarized epithelium affect apical membrane size and cilia [0.03%]
细胞分裂后的胞质分裂失败会影响顶膜大小和纤毛分布
Kaela S Lettieri,Katrina C McNeely,Noelle D Dwyer
Kaela S Lettieri
Cytokinetic abscission genes are linked to cancers and developmental disorders, but the consequences of disrupted abscission in vivo remain under-explored. Previously we showed that in the forebrain of Cep55 knockout (KO) mouse embryos, a s...
Ligase-dependent and independent functions of the C-terminus of Mms21 contribute to optimal growth and genome stability in Saccharomyces cerevisiae [0.03%]
Mms21 C端的连接酶依赖和非依赖功能可优化酿酒酵母的生长并维持基因组稳定性
Cheung Li,Anny Vo,Nkechinye Baadi et al.
Cheung Li et al.
An evolutionarily conserved E3 SUMO ligase, Mms21, orchestrates genome integrity processes. Our study examined a mutant of Saccharomyces cerevisiae Mms21, analogous to a mutant identified in a rare human condition characterized by genome in...
Asymmetric branched F-actin networks at apical clathrin coated pits support microvillar biogenesis [0.03%]
顶帽网格蛋白包被小窝的不对称分支F肌动蛋白网络支持微绒毛发生
Olivia Perkins,Malcom Díaz García,Matthew J Tyska
Olivia Perkins
Microvilli are conserved actin-based protrusions that expand surface area and absorptive capacity in epithelial tissues. In humans, the intestinal tract grows three-hundred trillion microvilli per day, yet the molecular mechanisms defining ...