Reprogramming Urine-Derived Cells using Commercially Available Self-Replicative RNA and a Single Electroporation [0.03%]
使用商业自复制RNA和单次电穿孔对尿液衍生细胞进行重编程
Marga J Bouma,Christiaan H Arendzen,Christine L Mummery et al.
Marga J Bouma et al.
We describe a protocol for efficient generation of human-induced pluripotent stem cells (hiPSCs) from urine-derived cells (UDCs) obtained from adult donors using self-replicative RNA containing the reprogramming factors OCT3/4, SOX2, KLF4, ...
Human iPSC-Derived Blood-Brain Barrier Models: Valuable Tools for Preclinical Drug Discovery and Development? [0.03%]
人iPSC来源的血脑屏障模型：预临床药物研发中的重要工具？
Antje Appelt-Menzel,Sabrina Oerter,Sanjana Mathew et al.
Antje Appelt-Menzel et al.
Translating basic biological knowledge into applications remains a key issue for effectively tackling neurodegenerative, neuroinflammatory, or neuroendocrine disorders. Efficient delivery of therapeutics across the neuroprotective blood-bra...
Methods for Automated Single Cell Isolation and Sub-Cloning of Human Pluripotent Stem Cells [0.03%]
人多能干细胞自动化单细胞分离和亚克隆的方法
Valeria Fernandez Vallone,Narasimha Swamy Telugu,Iris Fischer et al.
Valeria Fernandez Vallone et al.
Advances in human pluripotent stem cell (hPSC) techniques have led them to become a widely used and powerful tool for a vast array of applications, including disease modeling, developmental studies, drug discovery and testing, and emerging ...
From Hair to iPSCs-A Guide on How to Reprogram Keratinocytes and Why [0.03%]
从头发到iPSCs-关于如何重编程角质形成细胞的指南及原因
Stefanie Klingenstein,Moritz Klingenstein,Alexander Kleger et al.
Stefanie Klingenstein et al.
Keratinocytes, as a primary somatic cell source, offer exceptional advantages compared to fibroblasts, which are commonly used for reprogramming. Keratinocytes can beat fibroblasts in reprogramming efficiency and reprogramming time and, in ...
Simple Workflow and Comparison of Media for hPSC-Cardiomyocyte Cryopreservation and Recovery [0.03%]
hPSC-心脏肌肉细胞冷冻保存和恢复的简单流程及介质比较
Duncan C Miller,Carolin Genehr,Narasimha S Telugu et al.
Duncan C Miller et al.
Great progress has been made with protocols for the differentiation and functional application of hPSC-cardiomyocytes (hPSC-CMs) in recent years; however, the cryopreservation and recovery of hPSC-CMs still presents challenges and few repor...
Cell Banking of hiPSCs: A Practical Guide to Cryopreservation and Quality Control in Basic Research [0.03%]
hiPSC细胞库：冷冻保存和基础研究中质量控制的实际操作指南
Aya Shibamiya,Elisabeth Schulze,Dana Krauß et al.
Aya Shibamiya et al.
The reproducibility of stem cell research relies on the constant availability of quality-controlled cells. As the quality of human induced pluripotent stem cells (hiPSCs) can deteriorate in the course of a few passages, cell banking is key ...
Scaled Isolation of Mesenchymal Stem/Stromal Cell-Derived Extracellular Vesicles [0.03%]
间充质干细胞/ Stromal细胞衍生的外泌体的比例隔离
Verena Börger,Simon Staubach,Robin Dittrich et al.
Verena Börger et al.
Mesenchymal stem/stromal cells (MSCs) provide therapeutic effects in many diseases. Contrary to initial hypotheses, they act in a paracrine rather than a cellular manner. To this end, extracellular vesicles (EVs) have been found to mediate ...
Differentiation Protocol for 3D Retinal Organoids, Immunostaining and Signal Quantitation [0.03%]
三维视网膜类器官的分化协议、免疫染色及信号定量
Hannah Döpper,Julia Menges,Morgane Bozet et al.
Hannah Döpper et al.
Structures resembling whole organs, called organoids, are generated using pluripotent stem cells and 3D culturing methods. This relies on the ability of cells to self-reorganize after dissociation. In combination with certain supplemented f...
Advancing Stem Cell Technologies and Applications: A Special Collection from the PluriCore Network in the German Stem Cell Network (GSCN) [0.03%]
干细胞技术与应用的进步：德国干细胞网络(GSCN)中PluriCore网络的特别文集
Daniel Besser
Daniel Besser
Current Protocols in Stem Cell Biology is publishing a special collection of eight articles from members of the PluriCore Network in the German Stem Cell Network. © 2020 Wiley Periodicals LLC. ...
Efficient Multi-Allelic Genome Editing of Primary Cell Cultures via CRISPR-Cas9 Ribonucleoprotein Nucleofection [0.03%]
通过CRISPR-Cas9核糖核蛋白核转染有效编辑原代培养细胞的多等位基因基因组
Pia Hoellerbauer,Megan Kufeld,Patrick J Paddison
Pia Hoellerbauer
CRISPR-Cas9-based technologies have revolutionized experimental manipulation of mammalian genomes. However, limitations regarding the delivery and efficacy of these technologies restrict their application in primary cells. This article desc...