Xeno-free production of human embryonic stem cells in stirred microcarrier systems using a novel animal/human-component-free medium [0.03%]
无异种成分的人胚胎干细胞微载体搅拌悬浮培养系统的建立及其新型动物/人组分完全化学定义培养基的开发
Paulo André Nobrega Marinho,Daniel Tait Vareschini,Ismael Carlos Gomes et al.
Paulo André Nobrega Marinho et al.
Currently, stem cell research faces a major bottleneck related to the low efficiency of methods to produce large quantities of human embryonic stem cells (ESC) for use in clinical trials. Most culture media currently employed for human ESC ...
Radial-flow bioreactor enables uniform proliferation of human mesenchymal stem cells throughout a three-dimensional scaffold [0.03%]
径向流生物反应器可使人类间充质干细胞在整个三维支架中均匀增殖
Aiko Katayama,Taichi Arano,Toru Sato et al.
Aiko Katayama et al.
Mesenchymal stem cells (MSCs) obtained from human bone marrow are pluripotent and have been expanded and differentiated into several kinds of mesodermal tissue in vitro. To create bioartificial tissues and organs for implantation, it is nec...
Upcyte® microvascular endothelial cells repopulate decellularized scaffold [0.03%]
UPCYTE微血管内皮细胞在脱细胞支架上的再植生长
Katharina Scheller,Iris Dally,Nadja Hartmann et al.
Katharina Scheller et al.
A general problem in tissue engineering is the poor and insufficient blood supply to guarantee tissue cell survival as well as physiological tissue function. To address this limitation, we have developed an in vitro vascularization model in...
Benefits of spine stabilization with biodegradable scaffolds in spinal cord injured rats [0.03%]
可降解支架固定对脊髓损伤修复的积极作用研究
Nuno A Silva,Rui A Sousa,Joana S Fraga et al.
Nuno A Silva et al.
Spine stabilization upon spinal cord injury (SCI) is a standard procedure in clinical practice, but rarely employed in experimental models. Moreover, the application of biodegradable biomaterials for this would come as an advantage as it wo...
Characterization of dental epithelial stem cells from the mouse incisor with two-dimensional and three-dimensional platforms [0.03%]
小鼠前臼齿牙上皮干细胞的二维及三维生物学行为特征研究
Miquella G Chavez,Wenli Yu,Brian Biehs et al.
Miquella G Chavez et al.
Dental epithelial stem cells (DESCs) drive continuous growth in the adult mouse incisors. To date, a robust system for the primary culture of these cells has not been reported, and little is known about the basic molecular architecture of t...
Optimization and scale-up culture of human endometrial multipotent mesenchymal stromal cells: potential for clinical application [0.03%]
人子宫内膜多功能间充质干细胞的优化及放大培养：临床应用潜力
Gayathri Rajaraman,Jacinta White,Ker Sin Tan et al.
Gayathri Rajaraman et al.
We have previously identified and purified multipotent mesenchymal stromal cell (MSC)-like cells in the highly regenerative endometrial lining of the human uterus (eMSC) as CD140b⁺CD146⁺ cells. Due to ease of accessibility with minimal mo...
Continuous and uninterrupted oxygen tension influences the colony formation and oxidative metabolism of human mesenchymal stem cells [0.03%]
连续而恒定的氧张力影响人源间充质干细胞集落形成及氧化代谢
Girish Pattappa,Stephen D Thorpe,Nick C Jegard et al.
Girish Pattappa et al.
Mesenchymal stem cells (MSCs) are an attractive cell source for tissue engineering applications due to their multipotentiality and increased expansion potential compared to mature cells. However, the full potential of MSCs for cellular ther...
Improved function and growth of pancreatic cells in a three-dimensional bioreactor environment [0.03%]
三维生物反应器环境中胰腺细胞功能和生长的改善
Lisa Samuelson,David A Gerber
Lisa Samuelson
Methods of three-dimensional (3D) cell culture have made significant progress in recent years due to a better understanding of cell to cell interactions and the cell's interface with their surrounding environment. We hypothesized that a mic...
Two-dimensional polymer-based cultures expand cord blood-derived hematopoietic stem cells and support engraftment of NSG mice [0.03%]
基于二维聚合物的培养基能够扩增脐带血造血干细胞并支持植入NSG小鼠体内
Mónica Sofia Ventura Ferreira,Rebekka Kramann Schneider,Wolfgang Wagner et al.
Mónica Sofia Ventura Ferreira et al.
Currently, ex vivo expansion of hematopoietic stem cells (HSC) is still insufficient. Traditional approaches for HSC expansion include the use of stromal cultures, growth factors, and/or bioreactors. Biomaterial-based strategies provide new...
Three-dimensional cell morphometry for the quantification of cell-substrate interactions [0.03%]
三维细胞形态计量学在量化细胞-基底相互作用中的应用
Michael Shaw,Nilofar Faruqui,Elzbieta Gurdak et al.
Michael Shaw et al.
The initial attachment of cells to biomaterials is an important indicator of longer term cell-substrate biocompatibility. To study and quantify this interaction, we have developed a protocol for measuring temporal changes in the three-dimen...