Differentiation of human embryonic stem cells using size-controlled embryoid bodies and negative cell selection in the production of photoreceptor precursor cells [0.03%]
利用大小控制型胚胎样体和负向细胞筛选技术诱导人胚胎干细胞向视网膜感光神经元方向分化的研究
Anat Yanai,Christopher R J Laver,Aaron W Joe et al.
Anat Yanai et al.
We proposed to optimize the retinal differentiation protocols for human embryonic stem cells (hESCs) by improving cell handling. To improve efficiency, we first focused on the production of just one retinal precursor cell type (photorecepto...
Development of an improved three-dimensional in vitro intestinal mucosa model for drug absorption evaluation [0.03%]
一种改进的体外三维肠道黏膜模型的建立及其在药物吸收评价中的应用研究
Na Li,Dandan Wang,Zhigang Sui et al.
Na Li et al.
Human epithelial cell culture models of monolayer Caco-2 cells have been widely employed to assess the absorption of drug molecules across intestinal mucosa. However, cautions should be taken when interpreting the conclusions from those mod...
Noninvasive real-time monitoring by alamarBlue(®) during in vitro culture of three-dimensional tissue-engineered bone constructs [0.03%]
用于体外三维组织工程骨构建培养的非侵入式实时监测的alamarBlue(®)法
Xiaohua Zhou,Inge Holsbeeks,Saartje Impens et al.
Xiaohua Zhou et al.
Bone tissue engineering (TE) aims to develop reproducible and predictive three-dimensional (3D) TE constructs, defined as cell-seeded scaffolds produced by a controlled in vitro process, to heal or replace damaged and nonfunctional bone. To...
One-stage tissue engineering of bladder wall patches for an easy-to-use approach at the surgical table [0.03%]
一种简便的用于修补膀胱壁的一阶段组织工程法
Fatemeh Ajalloueian,Said Zeiai,Ramiro Rojas et al.
Fatemeh Ajalloueian et al.
We present a method for producing a cell-scaffold hybrid construct at the bedside. The construct is composed of plastic-compressed collagen together with a poly(ɛ-caprolactone) (PCL)-knitted mesh that yields an integrated, natural-syntheti...
A semiautomatic cell counting tool for quantitative imaging of tissue engineering scaffolds [0.03%]
用于组织工程支架定量成像的半自动细胞计数工具
Sebastian De Boodt,Ahmad Poursaberi,Jan Schrooten et al.
Sebastian De Boodt et al.
Automatic image analysis algorithms are in general dedicated quantification tools used for very specific types of microscopic cell images, but are not robust enough to accurately quantify the cell number and distribution in the wide variety...
Sex steroids regulate epithelial-stromal cell cross talk and trophoblast attachment invasion in a three-dimensional human endometrial culture system [0.03%]
性激素通过三维人子宫内膜培养系统调节上皮-间质细胞相互作用和滋养层细胞的黏附侵袭
Hai Wang,Silvina Bocca,Sandra Anderson et al.
Hai Wang et al.
Human embryo implantation involves a complex network of molecular signaling that is modulated by endocrine and paracrine pathways. Here, we performed studies using a unique and recently developed three-dimensional (3D) implantation model, c...
Monica L Moya,Yu-Hsiang Hsu,Abraham P Lee et al.
Monica L Moya et al.
Replicating in vitro the complex in vivo tissue microenvironment has the potential to transform our approach to medicine and also our understanding of biology. In order to accurately model the 3D arrangement and interaction of cells and ext...
Control of collagen production in mouse chondrocytes by using a combination of bone morphogenetic protein-2 and small interfering RNA targeting Col1a1 for hydrogel-based tissue-engineered cartilage [0.03%]
基于透明质酸水凝胶的软骨组织工程技术中I型胶原的基因沉默及胶原合成的BMP-2诱导调控研究
Emeline Perrier-Groult,Marielle Pasdeloup,Marilyne Malbouyres et al.
Emeline Perrier-Groult et al.
Because articular cartilage does not self-repair, tissue-engineering strategies should be considered to regenerate this tissue. Autologous chondrocyte implantation is already used for treatment of focal damage of articular cartilage. Unfort...
Assembly of a three-dimensional multitype bronchiole coculture model using magnetic levitation [0.03%]
一种基于磁力操控技术构建三维多类型终末细支气管上皮模型的方法
Hubert Tseng,Jacob A Gage,Robert M Raphael et al.
Hubert Tseng et al.
A longstanding goal in biomedical research has been to create organotypic cocultures that faithfully represent native tissue environments. There is presently great interest in representative culture models of the lung, which is a particular...
Magnetic targeting of human peripheral blood CD133+ cells for skeletal muscle regeneration [0.03%]
磁性靶向人体外周血CD133阳性细胞以促进肌肉再生
Shingo Ohkawa,Naosuke Kamei,Goki Kamei et al.
Shingo Ohkawa et al.
Skeletal muscle injuries often leave lasting functional damage or pain. Muscle injuries are routinely treated conservatively, but the most effective treatment to promote the repair of injured muscles has not yet been established. Our previo...