Generation of mouse induced pluripotent stem cells by protein transduction [0.03%]
蛋白质传递法诱导小鼠多能干细胞的产生
Csilla Nemes,Eszter Varga,Zsuzsanna Polgar et al.
Csilla Nemes et al.
Somatic cell reprogramming has generated enormous interest after the first report by Yamanaka and his coworkers in 2006 on the generation of induced pluripotent stem cells (iPSCs) from mouse fibroblasts. Here we report the generation of sta...
Mild heat stress enhances angiogenesis in a co-culture system consisting of primary human osteoblasts and outgrowth endothelial cells [0.03%]
轻度热应激通过人骨细胞和内皮细胞共培养体系促进血管生成
Ming Li,Sabine Fuchs,Thomas Böse et al.
Ming Li et al.
The repair and regeneration of large bone defects, including the formation of functional vasculature, represents a highly challenging task for tissue engineering and regenerative medicine. Recent studies have shown that vascularization and ...
Amniotic membrane scaffolds enable the development of tissue-engineered urothelium with molecular and ultrastructural properties comparable to that of native urothelium [0.03%]
胎盘膜支架使组织工程尿路上皮的分子和亚微观结构接近天然上皮体
Urška Dragin Jerman,Peter Veranič,Mateja Erdani Kreft
Urška Dragin Jerman
The amniotic membrane (AM) is a naturally derived biomaterial that possesses biological and mechanical properties of great importance for tissue engineering. The aim of our study was to determine whether the AM enables the formation of a no...
Novel and simple alternative to create nanofibrillar matrices of interest for tissue engineering [0.03%]
一种用于组织工程的纳米纤维基质的新而简单的替代品
Jérôme Sohier,Pierre Corre,Christophe Perret et al.
Jérôme Sohier et al.
Synthetic analogs to natural extracellular matrix (ECM) at the nanometer level are of great potential for regenerative medicine. This study introduces a novel and simple method to produce polymer nanofibers and evaluates the properties of t...
Effects of continuous passaging on mineralization of MC3T3-E1 cells with improved osteogenic culture protocol [0.03%]
改进的成骨培养体系中连续传代对MC3T3-E1细胞矿化的影响
Xiang-Zhen Yan,Wanxun Yang,Fang Yang et al.
Xiang-Zhen Yan et al.
The murine-derived MC3T3-E1 cell line provided by the American Type Culture Collection (ATCC) is a well-known osteogenic cell culture model system to test materials in vitro. However, the effect of passaging on its mineralization capacity h...
Natural corneal cell-based microenvironment as prerequisite for balanced 3D corneal epithelial morphogenesis: a promising animal experiment-abandoning tool in ophthalmology [0.03%]
自然角膜细胞微环境是三维角膜上皮发生及仿生构建的先决条件：有望成为眼科领域放弃动物实验的技术路径
Simon Schulz,David Beck,Dougal Laird et al.
Simon Schulz et al.
To achieve durable recognition as a promising animal experiment-abandoning tool in ophthalmology, in vitro engineered tissue equivalents of the human cornea should exhibit proper morphogenesis. Regarding this issue, we were seeking for the ...
Polyglycolic acid-polylactic acid scaffold response to different progenitor cell in vitro cultures: a demonstrative and comparative X-ray synchrotron radiation phase-contrast microtomography study [0.03%]
聚甘醇酸-聚乳酸支架体外不同祖细胞培养的反应：一种演示和比较的研究-X射线同步加速器相衬微层析法研究
Alessandra Giuliani,Francesca Moroncini,Serena Mazzoni et al.
Alessandra Giuliani et al.
Spatiotemporal interactions play important roles in tissue development and function, especially in stem cell-seeded bioscaffolds. Cells interact with the surface of bioscaffold polymers and influence material-driven control of cell differen...
Comparative Study
Tissue engineering. Part C, Methods. 2014 Apr;20(4):308-16. DOI:10.1089/ten.TEC.2013.0213 2014
Janina Burk,Ina Erbe,Dagmar Berner et al.
Janina Burk et al.
The use of decellularized tendon tissue as a scaffold for tendon tissue engineering provides great opportunities for future clinical and current research applications. The aim of this study was to assess the effect of repetitive freeze-thaw...
Transportation conditions for prompt use of ex vivo expanded and freshly harvested clinical-grade bone marrow mesenchymal stromal/stem cells for bone regeneration [0.03%]
临床级骨髓间充质基质/干细胞的运输条件以用于骨再生
Elena Veronesi,Alba Murgia,Anna Caselli et al.
Elena Veronesi et al.
Successful preliminary studies have encouraged a more translational phase for stem cell research. Nevertheless, advances in the culture of human bone marrow-derived mesenchymal stromal/stem cells (hBM-MSC) and osteoconductive qualities of c...
Tissue-engineered fibrin-based heart valve with a tubular leaflet design [0.03%]
基于组织工程纤维蛋白的心瓣膜管状瓣叶设计
Miriam Weber,Eriona Heta,Ricardo Moreira et al.
Miriam Weber et al.
The general approach in heart valve tissue engineering is to mimic the shape of the native valve in the attempt to recreate the natural haemodynamics. In this article, we report the fabrication of the first tissue-engineered heart valve (TE...