Increased osmolarity and cell clustering preserve canine notochordal cell phenotype in culture [0.03%]
高渗培养及细胞聚集有利于犬椎间盘未分化性细胞表型的维持
Sandra Spillekom,Lucas A Smolders,Guy C M Grinwis et al.
Sandra Spillekom et al.
Degeneration of the intervertebral disc (IVD) is associated with a loss of notochordal cells (NCs) from the nucleus pulposus (NP) and their replacement by chondrocyte-like cells. NCs are known to maintain extracellular matrix quality and st...
Development of 3D dynamic flow model of human liver and its application to prediction of metabolic clearance of 7-ethoxycoumarin [0.03%]
人肝三维动态流体模型的发展及其在预测7-乙氧基香豆素代谢清除率中的应用研究
Kyoungju Choi,William P Pfund,Melvin E Andersen et al.
Kyoungju Choi et al.
Primary and cryopreserved hepatocytes and immortalized hepatic cell lines in static two-dimensional monolayer culture format have been widely used as in vitro liver models for studies of xenobiotic metabolism, enzyme induction, hepatocyte r...
Wenting Zhang,Woo Y Lee,David S Siegel et al.
Wenting Zhang et al.
In vitro culturing of primary multiple myeloma cells (MMC) has been a major challenge as this plasma cell malignancy depends on the bone marrow environment for its survival. Using a microfluidic platform to emulate the dynamic physiology of...
Emily E Coates,John P Fisher
Emily E Coates
Recent cartilage engineering efforts have focused on development of zonally organized tissue. However, there remains a need for protocols that differentiate progenitor populations into chondrocytes of zonal phenotype. Here, we evaluate the ...
Mechanical characterization of tissue-engineered cartilage using microscopic magnetic resonance elastography [0.03%]
基于微观磁共振弹性技术的人工软骨力学表征研究
Ziying Yin,Thomas M Schmid,Temel K Yasar et al.
Ziying Yin et al.
Knowledge of mechanical properties of tissue-engineered cartilage is essential for the optimization of cartilage tissue engineering strategies. Microscopic magnetic resonance elastography (μMRE) is a recently developed MR-based technique t...
Anke Hoppensack,Christian C Kazanecki,David Colter et al.
Anke Hoppensack et al.
Human in vitro-manufactured tissue and organ models can serve as powerful enabling tools for the exploration of fundamental questions regarding cell, matrix, and developmental biology in addition to the study of drug delivery dynamics and k...
Rapid assessment of migration and proliferation: a novel 3D high-throughput platform for rational and combinatorial screening of tissue-specific biomaterials [0.03%]
一种新颖的三维高通量平台快速评估细胞迁移和增殖：用于组织特异性生物材料理性筛选及组合筛选
Courtney M Dumont,Pankaj Karande,Deanna M Thompson
Courtney M Dumont
Designing an ideal biomaterial supportive of multicellular tissue repair is challenging, especially with a poor understanding of the synergy between constituent proteins and growth factors. A brute-force approach, based on screening all pos...
The use of optical clearing and multiphoton microscopy for investigation of three-dimensional tissue-engineered constructs [0.03%]
光学清除和多光子显微镜在三维组织工程构建体研究中的应用
Elizabeth A Calle,Sam Vesuna,Sashka Dimitrievska et al.
Elizabeth A Calle et al.
Recent advances in three-dimensional (3D) tissue engineering have concomitantly generated a need for new methods to visualize and assess the tissue. In particular, methods for imaging intact volumes of whole tissue, rather than a single pla...
Quantitative, label-free characterization of stem cell differentiation at the single-cell level by broadband coherent anti-Stokes Raman scattering microscopy [0.03%]
基于宽带相干反斯托克斯拉曼散射显微镜的单细胞水平诱导多能干细胞分化过程无标记定量分析研究
Young Jong Lee,Sebastián L Vega,Parth J Patel et al.
Young Jong Lee et al.
We use broadband coherent anti-Stokes Raman scattering (BCARS) microscopy to characterize lineage commitment of individual human mesenchymal stem cells cultured in adipogenic, osteogenic, and basal culture media. We treat hyperspectral imag...
Characterization of a human skin equivalent model to study the effects of ultraviolet B radiation on keratinocytes [0.03%]
人皮肤等效模型的特征分析用于研究紫外线B辐射对角质形成细胞的影响
Tara L Fernandez,Derek R Van Lonkhuyzen,Rebecca A Dawson et al.
Tara L Fernandez et al.
The incidences of skin cancers resulting from chronic ultraviolet radiation (UVR) exposure are on the incline in both Australia and globally. Hence, the cellular and molecular pathways that are associated with UVR-induced photocarcinogenesi...