Tissue engineering: selecting the optimal fixative for immunohistochemistry [0.03%]
组织工程学：选择最佳的免疫组化固定液
Sabine Koch,Nadine Stappenbeck,Christian G Cornelissen et al.
Sabine Koch et al.
Background: In the immunohistochemical analysis of tissue-engineered structures, aggressive treatments for fixation and antigen retrieval can impair the quality of specimen staining and visualization. ...
Developmental cues for bone formation from parathyroid hormone and parathyroid hormone-related protein in an ex vivo organotypic culture system of embryonic chick femora [0.03%]
胚胎鸡股骨的外源性有机型培养系统中甲状旁腺激素和相关蛋白对骨形成的刺激信号
Emma L Smith,Janos M Kanczler,Carol A Roberts et al.
Emma L Smith et al.
Enhancement and application of our understanding of skeletal developmental biology is critical to developing tissue engineering approaches to bone repair. We propose that use of the developing embryonic femur as a model to further understan...
The adjuvant use of stromal vascular fraction and platelet-rich fibrin for autologous adipose tissue transplantation [0.03%]
富含血小板纤维蛋白和间质血管成分辅助自体脂肪移植的研究
Bin Liu,Xin-Ying Tan,Yan-Pu Liu et al.
Bin Liu et al.
Autologous adipose transplantation is rapidly gaining popularity for the restoration of soft tissue defects and lipoatrophy as well as for aesthetic improvements (e.g., facial reconstruction and rejuvenation). However, the current technique...
Pedro F Costa,Ana F Dias,Rui L Reis et al.
Pedro F Costa et al.
The aim of this work was to study the effect of cryopreservation over the functionality of tissue-engineered constructs, analyzing the survival and viability of cells seeded, cultured, and cryopreserved onto 3D scaffolds. Further, it also e...
Human platelet lysate gel provides a novel three dimensional-matrix for enhanced culture expansion of mesenchymal stromal cells [0.03%]
人血小板裂解液凝胶可作为增强间充质基质细胞三维培养扩增的新材料
Gudrun Walenda,Hatim Hemeda,Rebekka K Schneider et al.
Gudrun Walenda et al.
Cell culture in regenerative medicine needs to facilitate efficient expansion according to good manufacturing practice requirements. Human platelet lysate (HPL) can be used as a substitute for fetal calf serum without the risk of xenogeneic...
Macrocompression and nanoindentation of soft viscoelastic biological materials [0.03%]
软粘弹性生物材料的宏观压缩与纳米压入
V Timothy Nayar,James D Weiland,Andrea M Hodge
V Timothy Nayar
The study of the mechanical behavior of soft biological materials presents many challenges due to the materials' time-dependent mechanical response as well as inherent size and shape limitations. In this study, by using agar as a surrogate ...
Adipocyte induction of preadipocyte differentiation in a gradient chamber [0.03%]
脂肪细胞在浓度梯度室中诱导前脂肪细胞分化
Ning Lai,James K Sims,Noo Li Jeon et al.
Ning Lai et al.
Adipose tissue expansion involves enlargement of mature adipocytes and the formation of new adipocytes through the differentiation of locally resident preadipocytes. Factors released by the enlarged adipocytes are potential cues that induce...
Human skin model containing melanocytes: essential role of keratinocyte growth factor for constitutive pigmentation-functional response to α-melanocyte stimulating hormone and forskolin [0.03%]
含有黑素细胞的人工皮肤模型：角质细胞生长因子对基底黑素形成及促黑激素和 forskolin 刺激的黑素应答的必要性
Christine Duval,Corinne Chagnoleau,Florence Pouradier et al.
Christine Duval et al.
To study human skin pigmentation in a physiological in vitro model, we developed a pigmented reconstructed skin reproducing the three-dimensional architecture of the melanocyte environment and the interactions of melanocyte with its cellula...
Noninvasive, quantitative, spatiotemporal characterization of mineralization in three-dimensional collagen hydrogels using high-resolution spectral ultrasound imaging [0.03%]
基于高空间分辨光谱超声成像的三维胶原凝胶矿化非侵入定量表征研究
Madhu Gudur,Rameshwar R Rao,Yi-Sing Hsiao et al.
Madhu Gudur et al.
As tissue engineering products move toward the clinic, nondestructive methods to monitor their development and ensure quality are needed. In this study, high-resolution spectral ultrasound imaging (SUSI) was used to noninvasively characteri...
Charged nanomatrices as efficient platforms for modulating cell adhesion and shape [0.03%]
带电纳米矩阵作为调节细胞粘附和形状的有效平台
Jangho Kim,Deok-Ho Kim,Ki Taek Lim et al.
Jangho Kim et al.
In this article, we describe the design and manipulation of charged nanomatrices and their application as efficient platforms for modulating cell behaviors. Using electrospraying technology and well designed biomaterials, poly(ɛ-caprolacto...