Kristen J Verhey,Neha Kaul,Virupakshi Soppina
Kristen J Verhey
Long-distance transport in eukaryotic cells is driven by molecular motors that move along microtubule tracks. Molecular motors of the kinesin superfamily contain a kinesin motor domain attached to family-specific sequences for cargo binding...
Roberto Dominguez,Kenneth C Holmes
Roberto Dominguez
Actin is the most abundant protein in most eukaryotic cells. It is highly conserved and participates in more protein-protein interactions than any known protein. These properties, along with its ability to transition between monomeric (G-ac...
High-pressure protein crystallography and NMR to explore protein conformations [0.03%]
利用高压蛋白质晶体学和核磁共振研究蛋白质构象
Marcus D Collins,Chae Un Kim,Sol M Gruner
Marcus D Collins
High-pressure methods for solving protein structures by X-ray crystallography and NMR are maturing. These techniques are beginning to impact our understanding of thermodynamic and structural features that define not only the protein's nativ...
Chromatin dynamics [0.03%]
染色质动态学
Michael R Hübner,David L Spector
Michael R Hübner
The expression patterns of many protein-coding genes are orchestrated in response to exogenous stimuli, as well as cell-type-specific developmental programs. In recent years, researchers have shown that dynamic chromatin movements and inter...
Antoine M van Oijen,Joseph J Loparo
Antoine M van Oijen
Replication of DNA is carried out by the replisome, a multiprotein complex responsible for the unwinding of parental DNA and the synthesis of DNA on each of the two DNA strands. The impressive speed and processivity with which the replisome...
Gottfried Otting
Gottfried Otting
Paramagnetic metal ions offer outstanding opportunities for protein studies by nuclear magnetic resonance (NMR) spectroscopy. The paramagnetic effects manifested in the NMR spectra provide powerful restraints for the determination of the th...
Kelli G Kline,Michael R Sussman
Kelli G Kline
Genetic, chemical, and environmental perturbations can all induce large changes in cellular proteomes, and research aimed at quantifying these changes are an important part of modern biology. Although improvements in the hardware and softwa...
Anders E Carlsson
Anders E Carlsson
The dynamic nature of actin in cells manifests itself constantly. Polymerization near the cell edge is balanced by depolymerization in the interior, externally induced actin polymerization is followed by depolymerization, and spontaneous os...
Harden McConnell
Harden McConnell
My research has included chemical physics, electron and NMR spectroscopy, membrane biophysics, and immunology. This research was curiosity driven as well as problem and technique oriented. A theoretical equation was developed for relating n...
Colin Echeverría Aitken,Alexey Petrov,Joseph D Puglisi
Colin Echeverría Aitken
Our current understanding of the mechanism of translation is based on nearly fifty years of biochemical and biophysical studies. This mechanism, which requires the ribosome to manipulate tRNA and step repetitively along the mRNA, implies mo...