Artificial Restriction DNA Cutter Using Nuclease S1 for Site-Selective Scission of Genomic DNA [0.03%]
利用核酸酶S1构建人工限制性内切酶实现基因组DNA的定点切割
Arivazhagan Rajendran,Narumi Shigi,Jun Sumaoka et al.
Arivazhagan Rajendran et al.
By combining a pair of pseudo-complementary peptide nucleic acids (pcPNAs) with S1 nuclease, a novel tool to cut DNA at a predetermined site can be obtained. Complementary pcPNAs invade the DNA duplex and base pair to each strand of a targe...
Facile Modifications at the C4 Position of Pyrimidine Nucleosides via In Situ Amide Activation with 1H-Benzotriazol-1-yloxy-tris(dimethyl-amino)phosphonium Hexafluorophosphate [0.03%]
通过1H-苯并三唑-1-氧基-三双(二甲氨基)磷六氟磷酸盐原位活化酰胺快速实现嘧啶核苷C4位修饰
Hari K Akula,Mahesh K Lakshman
Hari K Akula
Two approaches for C4 modifications of silyl-protected thymidine, 2'-deoxyuridine, and 3'-azido-2',3'-dideoxythymidine (AZT) are described. In both, nucleoside amide activation with 1H-benzotriazol-1-yloxy-tris(dimethylamino)phosphonium hex...
Synthesis and Characterization of Site-Specific O6 -Alkylguanine DNA-Alkyl Transferase-Oligonucleotide Crosslinks [0.03%]
特定位点O-烷基鸟嘌呤DNA-烷基转移酶-寡核苷酸交联物的合成与表征
Pratibha P Ghodke,Matthew E Albertolle,Kevin M Johnson et al.
Pratibha P Ghodke et al.
O6 -Alkylguanine DNA-alkyltransferase (AGT), a DNA repair protein, can form crosslinks with DNA. The AGT-DNA crosslinks are known to be mutagenic when AGT is heterologously expressed in Escherichia coli, as well as in mammalian cells. To un...
Pavlina Viskova,Daniel Krafcik,Lukas Trantirek et al.
Pavlina Viskova et al.
In-cell NMR spectroscopy is a unique tool that enables the study of the structure and dynamics of biomolecules as well as their interactions in the complex environment of living cells at near-to-atomic resolution. In this article, detailed ...
Label-Free Electrophoretic Mobility Shift Assay (EMSA) for Measuring Dissociation Constants of Protein-RNA Complexes [0.03%]
无标签电泳迁移率变动分析法（electrophoretic mobility shift assay, EMSA）测定蛋白-核糖核酸复合物的解离常数
Minguk Seo,Li Lei,Martin Egli
Minguk Seo
The electrophoretic mobility shift assay (EMSA) is a well-established method to detect formation of complexes between proteins and nucleic acids and to determine, among other parameters, equilibrium constants for the interaction. Mixtures o...
A Photocrosslinking-Based RNA Chemical Proteomics Approach to Profile m6 A-Regulated Protein-RNA Interactions [0.03%]
一种基于光交联的RNA化学蛋白质组学方法用于研究m6A修饰调控的蛋白- RNA互作谱图
A Emilia Arguello,Tharan Srikumar,Ralph E Kleiner
A Emilia Arguello
Post-transcriptional modifications play an important role in RNA biology. In particular, the addition of small chemical groups to the nucleobases of mRNA can affect how modified transcripts are processed in the cell, thereby impacting gene ...
Ruth Suchsland,Bettina Appel,Sabine Müller
Ruth Suchsland
We have developed two methods, in solution and on solid phase, that give easy access to trinucleotide phosphoramidites capable of undergoing coupling reactions by the solid-phase phosphoramidite approach. The solution protocol is characteri...
Synthesis of Fluorescence Turn-On DNA Hybridization Probe Using the DEA tC 2'-Deoxycytidine Analog [0.03%]
基于DEA-tC的荧光开启型DNA杂交探针的构建
M Benjamin Turner,Brooke A Anderson,George N Samaan et al.
M Benjamin Turner et al.
DEA tC is a tricyclic 2'-deoxycytidine analog that can be incorporated into oligonucleotides by solid-phase synthesis and that exhibits a large fluorescence enhancement when correctly base-paired with a guanine base in a DNA-DNA duplex. The...
Synthesis and Application of Interstrand Cross-Linked Duplexes by Covalently Linking a Pair of Abasic Sites [0.03%]
通过共价连接一对无嘌呤/嘧啶位点合成和应用链间交联的双链体
Yu Hirano,Naoshi Kojima,Yasuo Komatsu
Yu Hirano
Interstrand cross-linking of DNA or RNA inhibits the double strands from dissociating into single strands. This article contains detailed procedures for the synthesis of a novel interstrand cross-linker that comprises a bis-aminooxy naphtha...
Synthesis of Nucleoside-5'-O-Tetraphosphates from Activated Trimetaphosphate and Nucleoside-5'-O-Monophosphates [0.03%]
由激活的三元磷酸和核苷一磷酸合成核苷四磷酸
Samy Mohamady,Scott D Taylor
Samy Mohamady
This article describes a straight-forward chemical method for the synthesis of nucleoside-5'-O-tetraphosphates, such as cytosine-, guanosine-, adenosine-, and uridine-5'-O-tetraphosphates, starting from the corresponding nucleoside monophos...