Ziyan Zhang,Rajat Singh,Michael Aschner
Ziyan Zhang
Macroautophagy (hereafter referred to as autophagy) is a degradation pathway that delivers cytoplasmic materials to lysosomes via double-membraned vesicles designated autophagosomes. Cytoplasmic constituents are sequestered into autophagoso...
Multielectrode Array (MEA) Assay for Profiling Electrophysiological Drug Effects in Human Stem Cell-Derived Cardiomyocytes [0.03%]
人诱导多能干细胞源性心肌细胞电生理药物效应检测的多电极阵列(MEA)分析方法
Mike Clements
Mike Clements
More relevant and reliable preclinical cardiotoxicity tests are required to improve drug safety and reduce the cost of drug development. Human stem cell-derived cardiomyocytes (hSC-CMs) provide a potential model for the development of super...
Determination of Metabolic Viability and Cell Mass Using a Tandem Resazurin/Sulforhodamine B Assay [0.03%]
基于重氮树脂紫/硫黄素B联合检测的代谢活性及细胞质量测定方法研究
Filomena S G Silva,Irina G Starostina,Vilena V Ivanova et al.
Filomena S G Silva et al.
The identification of rapid, reliable, and highly reproducible biological assays that can be standardized and routinely used in preclinical tests constitutes a promising approach to reducing drug discovery costs and time. This unit details ...
Investigating the Effects of Particulate Matter on House Dust Mite and Ovalbumin Allergic Airway Inflammation in Mice [0.03%]
探究颗粒物对小鼠屋尘螨及卵清蛋白过敏性气道炎症的影响
Alejandro R Castañeda,Kent E Pinkerton
Alejandro R Castañeda
Particulate matter (PM), a component of air pollution, has been shown to enhance allergen-mediated airway hypersensitivity and inflammation. Surprisingly, exposure to PM during the sensitization to allergen is sufficient to produce immunolo...
PCR-Based Analysis of Mitochondrial DNA Copy Number, Mitochondrial DNA Damage, and Nuclear DNA Damage [0.03%]
基于PCR的线粒体DNA拷贝数、线粒体DNA损伤和核DNA损伤分析
Claudia P Gonzalez-Hunt,John P Rooney,Ian T Ryde et al.
Claudia P Gonzalez-Hunt et al.
Because of the role that DNA damage and depletion play in human disease, it is important to develop and improve tools to assess these endpoints. This unit describes PCR-based methods to measure nuclear and mitochondrial DNA damage and copy ...
Peace C Ezeh,Huan Xu,Shu Chun Wang et al.
Peace C Ezeh et al.
Development of blood cells through hematopoiesis occurs in the bone marrow (BM), and can be adversely impacted by various substances and/or conditions ranging from known therapeutic, intentionally administered xenobiotics to unintentional f...
Associating Changes in the Immune System with Clinical Diseases for Interpretation in Risk Assessment [0.03%]
基于临床疾病免疫系统变化的风险评估解释模型构建与应用研究
Jamie C DeWitt,Dori R Germolec,Robert W Luebke et al.
Jamie C DeWitt et al.
This overview is an update of the unit originally published in 2004. While the basic tenets of immunotoxicity have not changed in the past 10 years, several publications have explored the application of immunotoxicological data to the risk ...
The Use of Induced Pluripotent Stem Cells for the Study and Treatment of Liver Diseases [0.03%]
诱导性多能干细胞在肝病研究和治疗中的应用
Marc C Hansel,Julio C Davila,Massoud Vosough et al.
Marc C Hansel et al.
Liver disease is a major global health concern. Liver cirrhosis is one of the leading causes of death in the world and currently the only therapeutic option for end-stage liver disease (e.g., acute liver failure, cirrhosis, chronic hepatiti...
Kun He Lee,Michael Aschner
Kun He Lee
Response via noxious stimulus can be an important indicator of sensory neuron function and overall health of an organism. If the stimulation is quick and simple, and the animal can be rescued afterwards, such a method not only allows for as...
Use of Ciliogenesis to Detect Aneugens: The Role of Primary Cilia [0.03%]
通过基体和纤毛的形成来鉴定染色体非整倍体致突变物的作用机制研究
Kathyayini V Divi,Yvona Ward,Miriam C Poirier et al.
Kathyayini V Divi et al.
Primary cilia arise from the centrosomes of quiescent or post-mitotic cells, and serve as sensory organelles that communicate mechanical and chemical stimuli from the environment to the interior of the cell. Cilium formation may, therefore,...