Using Chromatin Immunoprecipitation in Toxicology: A Step-by-Step Guide to Increasing Efficiency, Reducing Variability, and Expanding Applications [0.03%]
染色质免疫沉淀在毒理学中的应用：逐步提高效率、减少可变性及扩展应用范围指南
Shaun D McCullough,Doan M On,Emma C Bowers
Shaun D McCullough
Histone modifications work in concert with DNA methylation to regulate cellular structure, function, and response to environmental stimuli. More than 130 unique histone modifications have been described to date, and chromatin immunoprecipit...
Preparation of Specific Compartments of the Lungs for Pathologic and Biochemical Analysis of Toxicologic Responses [0.03%]
毒理学反应的病理和生化分析的肺特异性隔室的制备
Laura S Van Winkle,Jacklyn S Kelty,Charles G Plopper
Laura S Van Winkle
This unit focuses on protocols for assessing microenvironment-specific responses in the thoracic lung tissues. Aspects of the entire respiratory system serve as potential targets for candidate toxicants, but each candidate toxicant may impa...
Flow-Cytometry-Based Method to Detect Escherichia coli and Shigella Spp. Using 16S rRNA-Based Probe [0.03%]
一种基于16SrRNA的针对大肠杆菌和志贺菌的流式细胞仪检测法
Yong Xue,Jon G Wilkes,Ted J Moskal et al.
Yong Xue et al.
Detection of microbial contamination in foods before they go on to the market can help prevent the occurrence of foodborne illness outbreaks. Current methods for the detection of Escherichia coli are limited by time-consuming procedures, wh...
Assessment of Bile Salt Export Pump (BSEP) Inhibition in Membrane Vesicles Using Radioactive and LC/MS-Based Detection Methods [0.03%]
基于放射性和LC/MS检测方法的胆盐出口泵(BSEP)抑制作用评估研究报告
Lisa D Marroquin,Paul D Bonin,Julie Keefer et al.
Lisa D Marroquin et al.
The bile salt export pump (BSEP, ABCB11) belongs to the ATP-binding-cassette superfamily of transporters and is predominately found in the liver. BSEP is an efflux transporter that plays a critical role in the secretion of bile salts into t...
Phillip A Wages
Phillip A Wages
Protein sulfenylation is a post-translational modification that is linked to many cell signaling networks and specific protein functions, thus the detection of any sulfenylated protein after a toxicological exposure is of importance. Specif...
Sadikshya Bhandari,Clare Melchiorre,Kristen Dostie et al.
Sadikshya Bhandari et al.
Metallothioneins (MTs) are small molecular weight stress response proteins that play a central role as reservoir of essential divalent heavy metal cations such as zinc and copper, and also can diminish the effects of toxic heavy metals such...
Fluorescence-Based Microplate Assays for In Vitro Assessment of Mitochondrial Toxicity, Metabolic Perturbation, and Cellular Oxygenation [0.03%]
基于荧光的微孔板测定法用于体外线粒体毒性、代谢紊乱和细胞氧合评估
James Hynes,Conn Carey,Yvonne Will
James Hynes
High-throughput in vitro cell metabolism assays are of particular use for identification and delineation of mitochondrial toxicity and related metabolic perturbation. Here, a panel of fluorescence-based metabolism assays are described for m...
Katy Phelan,Kristin M May
Katy Phelan
Cultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the ba...
Susanne Ramm,Melanie Adler,Vishal S Vaidya
Susanne Ramm
Kidney toxicity due to drugs and chemicals poses a significant health burden for patients and a financial risk for pharmaceutical companies. However, currently no sensitive and high-throughput in vitro method exists for predictive nephrotox...
In Vivo Determination of Mitochondrial Function Using Luciferase-Expressing Caenorhabditis elegans: Contribution of Oxidative Phosphorylation, Glycolysis, and Fatty Acid Oxidation to Toxicant-Induced Dysfunction [0.03%]
利用表达萤火虫荧光素酶的秀丽隐杆线虫体内测定线粒体功能：氧化磷酸化、糖酵解和脂肪酸氧化在线粒体毒物诱导的功能障碍中的贡献
Anthony L Luz,Cristina Lagido,Matthew D Hirschey et al.
Anthony L Luz et al.
Mitochondria are a target of many drugs and environmental toxicants; however, how toxicant-induced mitochondrial dysfunction contributes to the progression of human disease remains poorly understood. To address this issue, in vivo assays ca...