Use of Human Pluripotent Stem Cell Derived-Cardiomyocytes to Study Drug-Induced Cardiotoxicity [0.03%]
人多能干细胞源心脏细胞在药物致心肌毒理研究中的应用
Agnes Maillet,Kim Peng Tan,Liam R Brunham
Agnes Maillet
Drug-induced cardiotoxicity is the one of the most common causes of drug withdrawal from market. A major barrier in managing the risk of drug-induced cardiotoxicity has been the lack of relevant models to study cardiac safety. Human pluripo...
Kirk P Van Ness,Shih-Yu Chang,Elijah J Weber et al.
Kirk P Van Ness et al.
The liver and the kidney are key toxicity target organs during drug development campaigns, as they typically carry the burden of drug transport and metabolism. Primary hepatocytes and proximal tubule epithelial cells grown in traditional in...
Optimizing Dual Fluorescent Analysis to Investigate the Toxicity of AgNPs in E. coli [0.03%]
优化双荧光分析以研究AgNPs对E.coli的毒性作用
Wei Hong,Shaopeng Chen
Wei Hong
The ever-increasing use of silver nanoparticles (AgNPs) carries potential ecotoxicological risks. For full risk assessment, E. coli cells harboring a plasmid with a constitutively expressed GFP gene under control of lac promoter (lac::GFP) ...
3D Differentiation of LUHMES Cell Line to Study Recovery and Delayed Neurotoxic Effects [0.03%]
LUHMES细胞系的三维诱导分层及在研究神经恢复和毒性效应中的应用
Georgina Harris,Helena Hogberg,Thomas Hartung et al.
Georgina Harris et al.
Current neurotoxicity testing and the study of molecular mechanisms in neurodegeneration in vitro usually focuses on acute exposures to compounds. 3D Lund human mesencephalic (LUHMES) cells allow long-term treatment or pulse exposure in com...
Combining Cytotoxicity Assessment and Xenopus laevis Phenotypic Abnormality Assay as a Predictor of Nanomaterial Safety [0.03%]
结合细胞毒性评估和非洲爪蟾胚胎表型异常测定作为纳米材料安全性的预测手段
Karamallah Al-Yousuf,Carl A Webster,Grant N Wheeler et al.
Karamallah Al-Yousuf et al.
The African clawed frog, Xenopus laevis, has been used as an efficient pre-clinical screening tool to predict drug safety during the early stages of the drug discovery process. X. laevis is a relatively inexpensive model that can be used in...
Yu-Chi Chang,Toby B Cole,Lucio G Costa
Yu-Chi Chang
Autism spectrum disorder (ASD) represents a heterogeneous group of disorders characterized by alterations in three behavioral symptom domains: Social interactions, verbal and nonverbal communication, and repetitive behaviors. Increasing pre...
Micropatterned Co-Cultures of Human Hepatocytes and Stromal Cells for the Assessment of Drug Clearance and Drug-Drug Interactions [0.03%]
用于药物清除和药物相互作用评估的人肝细胞和间充质细胞微图案共培养体系
Christine Lin,Salman R Khetani
Christine Lin
Drug clearance rates from the body can determine drug exposure that can affect efficacy or toxicity. Thus, accurate prediction of drug clearance during preclinical development can help guide dose selection in humans, but animal testing is n...
Use of Primary Rat Hepatocytes for Prediction of Drug-Induced Mitochondrial Dysfunction [0.03%]
大鼠原代肝细胞用于预测药物引起的线粒体功能障碍
Cong Liu,Shuichi Sekine,Binbin Song et al.
Cong Liu et al.
Mitochondrial dysfunction plays a central role in drug-induced liver injury. To evaluate drug-induced mitochondrial impairment, several isolated mitochondria- or cell line-based assays have been reported. Among them, culturing HepG2 cells i...
A Multi-Parametric Fluorescent Assay for the Screening and Mechanistic Study of Drug-Induced Steatosis in Liver Cells in Culture [0.03%]
一种用于药物诱导的肝细胞脂肪变性的筛选和机理研究的多参数荧光分析法
Laia Tolosa,M José Gómez-Lechón,M Teresa Donato
Laia Tolosa
Human hepatic cells have been used for drug safety risk evaluations throughout early development phases. They provide rapid, cost-effective early feedback to identify drug candidates with potential hepatotoxicity. This unit presents a cell-...
Quantification of Lactate Dehydrogenase for Cell Viability Testing Using Cell Lines and Primary Cultured Astrocytes [0.03%]
基于细胞系和原代培养星形胶质细胞的乳酸脱氢酶定量检测细胞活力的方法
Simon Kaja,Andrew J Payne,Yuliya Naumchuk et al.
Simon Kaja et al.
Drug discovery heavily relies on cell viability studies to assess the potential toxicity of drug candidates. L-Lactate dehydrogenase (LDH) is a cytoplasmic enzyme that catalyzes the concomitant interconversions of pyruvate to L-lactate and ...