In Vitro Methods to Characterize the Effects of Tobacco and Nontobacco Products on Human Platelet Function [0.03%]
体外方法用于表征烟草和非烟草产品对人血小板功能的影响
Shannon G Loelius,Sherry L Spinelli,Katie L Lannan et al.
Shannon G Loelius et al.
In this document, we describe methods for the isolation, treatment, and functional testing of human blood platelets in vitro. Functional assays for inflammatory function include flow cytometry and immunoassays for platelet release of platel...
An In Vitro Model of Hematotoxicity: Differentiation of Bone Marrow-Derived Stem/Progenitor Cells into Hematopoietic Lineages and Evaluation of Lineage-Specific Hematotoxicity [0.03%]
一种血液毒性的体外模型：骨髓干细胞分化为各种血系及各系特异的血液毒性评价
Prathap Kumar Mahalingaiah,Tammy Palenski,Terry R Van Vleet
Prathap Kumar Mahalingaiah
Hematotoxicity is a significant issue for drug safety and can result from direct cytotoxicity toward circulating mature blood cell types as well as targeting of immature blood-forming stem cells/progenitor cells in the bone marrow. In vitro...
Quantification of Serum Voriconazole, Isavuconazole, and Posaconazole by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) [0.03%]
液相色谱串联质谱法测定伏立康唑、艾沙康唑和泊沙康唑血药浓度
Andrew Smith,Justin Dowis,Deborah French
Andrew Smith
Voriconazole, isavuconazole, and posaconazole are triazole anti-fungal drugs commonly used to treat invasive fungal infections. Therapeutic drug monitoring is commonly carried out when patients are prescribed these drugs to ensure that the ...
Effects of Rodent Diet Choice and Fiber Type on Data Interpretation of Gut Microbiome and Metabolic Disease Research [0.03%]
啮齿类动物饮食选择及纤维类型对肠道菌群和代谢性疾病研究的数据解读的影响
Michael A Pellizzon,Matthew R Ricci
Michael A Pellizzon
Poor diet reporting and improperly controlling laboratory animal diet continues to reduce our ability to interpret data effectively in animal studies. In order to make the best use of our resources and improve research transparency, proper ...
Generating Germ-Free Drosophila to Study Gut-Microbe Interactions: Protocol to Rear Drosophila Under Axenic Conditions [0.03%]
生成无菌果蝇以研究肠道微生物互作的培养无菌果蝇的操作流程
Christa Kietz,Vilma Pollari,Annika Meinander
Christa Kietz
As several diseases have been linked to dysbiosis of the human intestinal microflora, manipulation of the microbiota has emerged as an exciting new strategy for potentially treating and preventing diseases. However, the human microbiota con...
Anthony Flamier,Supriya Singh,Theodore P Rasmussen
Anthony Flamier
Human birth defects are relatively common and can be caused by exposure to environmental teratogens or to pharmaceuticals with teratogenic activities. Human embryonic stem cells (hESCs), by virtue of their pluripotent nature, provide an exc...
Determination of Mycophenolic Acid and Mycophenolic Acid Glucuronide Using Liquid Chromatography Tandem Mass Spectrometry (LC/MS/MS) [0.03%]
用高效液相色谱-串联质谱法测定霉酚酸和霉酚酸葡萄糖醛酸酯
Uttam Garg,Ada Munar,Clinton Frazee
Uttam Garg
Mycophenolic acid (MPA) is an immunosuppressant that is used in renal, liver, and heart transplantation. Due to its narrow therapeutic range, monitoring of MPA levels is essential to avoid toxicity and organ rejection. Although immunoassays...
A Real-Time Image-Based Approach to Distinguish and Discriminate Apoptosis from Necrosis [0.03%]
一种基于实时图像区分和鉴别凋亡与坏死的方法
Asha Lekshmi,Shankara Narayanan Varadarajan,Santhik Subhasingh Lupitha et al.
Asha Lekshmi et al.
Recent cell biology studies reveal that a cell can die through multiple pathways via distinct signaling mechanisms. Among these, apoptosis and necrosis are two distinct cell death pathways, and their detection and discrimination is vital in...
Evaluation of Marijuana Compounds on Neuroimmune Endpoints in Experimental Autoimmune Encephalomyelitis [0.03%]
大麻化合物对实验性自身免疫性脑脊髓炎中神经免疫指标的影响评估
Barbara L F Kaplan
Barbara L F Kaplan
Cannabinoid compounds refer to a group of more than 60 plant-derived compounds in Cannabis sativa, more commonly known as marijuana. Exposure to marijuana and cannabinoid compounds has been increasing due to increased societal acceptance fo...
Development of an In Vitro Method to Estimate the Sensitization Induction Level of Contact Allergens [0.03%]
体外接触致敏诱导水平估计方法的建立
Valentina Galbiati,Sue Gibbs,Erwin Roggen et al.
Valentina Galbiati et al.
Recently, an in vitro procedure, which combines the epidermal equivalent potency assay with assessment of IL-18 to provide a single test for identification and classification of skin sensitizers, was developed and validated. This unit will ...