Patrick Ziegler,Markus G Manz
Patrick Ziegler
By transplanting human hematopoietic stem and progenitor cells in newborn immunodeficient mice, researchers have recently achieved development of a human adaptive immune system, consisting of B cells, T cells, dendritic cells, and some cell...
Detection of the mitochondrial apoptosis-induced channel (MAC) and its regulation by Bcl-2 family proteins [0.03%]
线粒体凋亡通道(MAC)的检测及其与Bcl-2家族蛋白的作用关系研究
Kathleen W Kinnally,Sonia Martinez-Caballero,Laurent M Dejean
Kathleen W Kinnally
Apoptosis is a phenomenon fundamental to higher eukaryotes that is integral to such diverse cellular processes as tissue homeostasis, organogenesis, and response to toxins. The release from mitochondria of apoptotic factors such as cytochro...
Single-cell analysis of lipid rafts in lymphocytes and in T cell-containing immunoconjugates [0.03%]
淋巴细胞和T细胞免疫复合体中单细胞脂筏分析
William T Lee,Andrew R O Watson
William T Lee
Within the plasma membranes of many different cell types, certain membrane lipids, including cholesterol and sphingolipids, form lateral assemblies surrounded by unsaturated glycerophospholipids. The concentration of such membrane lipids an...
C B Klein,L Broday,M Costa
C B Klein
Mutagenesis assays in mammalian cells are frequently used to complement bacterial mutagenesis assays. This unit describes a mutagenesis assay using either Chinese hamster V79 cells or V79-derivative gpt transgenic cell line to assess the ef...
J P Plastaras,L J Marnett
J P Plastaras
Determining the levels of various DNA adducts has become an essential tool in understanding the toxicology of carcinogens. Direct measurement of DNA adduct levels, the true biologically effective dose of a mutagen, can be correlated with bi...
E Zeiger,K Mortelmans
E Zeiger
The Ames test is a widely accepted short-term assay for detecting chemicals that induce mutations in the DNA of organisms. The assay is a reverse mutation assay that detects the mutational reversion of his-dependent Salmonella to his-indepe...
James E Klaunig,Yuhui Shi
James E Klaunig
Gap junctions are important plasma membrane organelles through which most cells in normal tissues communicate with each other. They exist in two neighboring cells and each cell contributes half of the structure. One gap junction consists of...
Fluorescent pH and oxygen probes of the assessment of mitochondrial toxicity in isolated mitochondria and whole cells [0.03%]
用于评价分离线粒体和完整细胞中线粒体毒性的荧光pH和氧探针
James Hynes,Edward Natoli Jr,Yvonne Will
James Hynes
Oxygen consumption measurements provide information on the activity of the mitochondrial electron transport chain and such measurements can be carried out on both isolated mitochondria and whole cells. Additional information is available on...
Sashi Nadanaciva,Yvonne Will
Sashi Nadanaciva
Mitochondria generate most of the cell's ATP and play key roles in fatty acid oxidation, steroid synthesis, heme synthesis, thermogenesis, calcium homeostasis, and apoptosis. With the development of new methods to study mitochondrial functi...
Cross-species genetic toxicity assessment accomplished by flow cytometric analysis of blood [0.03%]
通过流式细胞分析血液完成跨物种遗传毒性评估
Jeffrey C Bemis,Dorothea K Torous,Carol R Tometsko et al.
Jeffrey C Bemis et al.
The formation of micronuclei in blood cells has been an established indicator of genotoxicity for decades. Standard microscopy methods are time-consuming and lack the objectivity that fully automated methods can provide. The ability of flow...