Human cytochrome P450: metabolism of testosterone by CYP3A4 and inhibition by ketoconazole [0.03%]
人类细胞色素P450：CYP3A4介导的睾丸素代谢及酮康唑的抑制作用
Khawja A Usmani,Jun Tang
Khawja A Usmani
This unit describes methods for measuring CYP3A4 activity using testosterone as a specific substrate, and for measuring CYP3A4 inhibition using ketoconazole as a selective inhibitor of testosterone oxidation. CYP3A4 is one of the most impor...
Determination of paraoxonase 1 status and genotypes at specific polymorphic sites [0.03%]
PARA氧化酶1状态及特定多态性位点基因型的测定
Rebecca J Richter,Rachel L Jampsa,Gail P Jarvik et al.
Rebecca J Richter et al.
The procedures for determining paraoxonase (PON1) status and for determining PON1 genotypes for polymorphisms in coding and important regulatory regions are described. PON1 status is determined by a functional two-substrate analysis of plas...
Techniques for measuring the activity of carboxylic acid:CoA ligase and acyl-CoA:amino acid N-acyltransferase: the amino acid conjugation pathway [0.03%]
羧酸：CoA连接酶和酰基-CoA：氨基酸N-酰基转移酶的测定技术：氨基酸结合途径
Michael Kelley,Donald A Vessey
Michael Kelley
A wide variety of xenobiotic carboxylic acids are metabolized to their amino acid conjugates via a pathway that exists primarily in liver and kidney. This conjugation occurs in a two-step pathway catalyzed by two distinct types of enzymes, ...
Randy L Rose
Randy L Rose
Measurement of Flavin-Containing Monooxygenase (FMO) Activities (Randy L. Rose, North Carolina State University, Raleigh, North Carolina). This unit describes methods used for measuring the presence of flavin-containing monooxygenases using...
Analysis of the aryl hydrocarbon receptor (AhR) signal transduction pathway [0.03%]
芳烃受体(AhR)信号转导途径分析研究进展
Michael S Denison,Jane M Rogers,S Renee Rushing et al.
Michael S Denison et al.
Analysis of the Ah Receptor Signal Transduction Pathway (Michael S. Denison, Jane M. Rohers, S. Renee Rushing. Carol L. Jones, Selwyna C. Tetangico, and Sharon Heath-Pagliuso, University of California, Davis, California).The protocols in th...
Masakiyo Hosokawa,Tetsuo Satoh
Masakiyo Hosokawa
Measurement of Carboxylesterase (CES) Activities (Masakiyo Hosokawa, Chiba University, Chiba, Japan and Tetsuo Satoh, Biomedical Research Institute, Chiba, Japan). Mammalian carboxyesterase (CES) efficiently catalyzes the hydrolysis of a va...
Charlene A McQueen
Charlene A McQueen
This unit describes methods for measuring the activity of arylamine N-acetyltransferases (NAT). Genetic polymorphisms in NAT 1 and NAT 2 have been associated with susceptibility to aromatic amines carcinogens and effects of therapeutic drug...
Assays for the classification of two types of esterases: carboxylic ester hydrolases and phosphoric triester hydrolases [0.03%]
用于酯酶分类的测定方法：羧酸酯水解酶和磷酸三酯水解酶
Douglas D Anspaugh,R Michael Roe
Douglas D Anspaugh
Assays for the Classification of Two Types of Esterases: Carboxylic Ester Hydrolase and Phosphoric Triester Hydrolase (Douglas D. Anspaugh and Michael Roe, North Carolina State University, Raleigh, North Carolina). This unit describes assay...
J J Sheng,V Sharma,M W Duffel
J J Sheng
Two methods are presented for determining the catalytic activity of aryl and alcohol sulfotransferases. The first assay is a simple and rapid procedure that is based on the extraction of a paired ion formed between the product organic sulfa...
Detection of metabolites using high-performance liquid chromatography and mass spectrometry [0.03%]
高效液相色谱与质谱技术在代谢产物检测中的应用
T D McClure
T D McClure
This unit provides a discussion of high-pressure liquid chromatography-atmospheric pressure ionization mass spectrometry (LC-API-MS) for separation, detection, and identification of products from xenobiotic metabolism. It includes an introd...