High-Throughput Assessment of Mechanistic Toxicity of Chemicals in Miniaturized 3D Cell Culture [0.03%]
基于微型三维细胞培养的化学品机制毒理学的高通量评估方法
Pranav Joshi,Soo-Yeon Kang,Akshata Datar et al.
Pranav Joshi et al.
High-content imaging (HCI) assays on two-dimensional (2D) cell cultures often do not represent in vivo characteristics accurately, thus reducing the predictability of drug toxicity/efficacy in vivo. On the other hand, conventional 3D cell c...
Assessment of Altered Cholesterol Homeostasis by Xenobiotics Using Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry [0.03%]
基于超高效液相色谱-串联质谱的胆固醇稳态紊乱评估方法学研究
Josi Herron,Kelly M Hines,Libin Xu
Josi Herron
Cholesterol and cholesterol-derived oxysterols are critical for embryonic development, synapse formation and function, and myelination, among other biological functions. Indeed, alterations in levels of cholesterol, sterol precursors, and o...
Simultaneous Determination of Uranium and Depleted Uranium Isotopic Ratio Using Inductively Coupled Mass Spectrometry [0.03%]
采用感应耦合质谱法同时测定铀和贫化铀同位素比值
Maria A Velez-Quinones,Hanna Xu,Nhu Vo et al.
Maria A Velez-Quinones et al.
We have developed and validated a method for the simultaneous quantitative measurement of total uranium (TU) and uranium 235 U/238 U isotopic ratio (UIR) in urine by inductively coupled plasma mass spectrometry (ICP-MS) using a Thermo Scien...
Determination of Thirteen Trace and Toxic Elements in Urine Using Inductively Coupled Mass Spectrometry [0.03%]
用电感耦合等离子体质谱法测定尿液中十三种痕量和有毒元素
Maria A Velez-Quinones,Hanna Xu,Nhu Vo et al.
Maria A Velez-Quinones et al.
We developed and validated a method for the assessment of thirteen separate trace and toxic elements using Inductively Coupled Plasma-Mass Spectrometry (ICP-MS). Included elements were as follows: aluminum, chromium, manganese, iron, cobalt...
Structural and Functional Analysis of the Gut Microbiome for Toxicologists [0.03%]
毒理学家的肠道微生态结构和功能分析
Robert G Nichols,Jingwei Cai,Iain A Murray et al.
Robert G Nichols et al.
Characterizing the reciprocal interactions between toxicants, the gut microbiota, and the host, holds great promise for improving our mechanistic understanding of toxic endpoints. Advances in culture-independent sequencing analysis (e.g., 1...
Analysis of Mitochondrial Protein Synthesis: De Novo Translation, Steady-State Levels, and Assembled OXPHOS Complexes [0.03%]
线粒体蛋白质合成分析：从头翻译、稳态水平和组装的氧化磷酸化复合物
Taru Hilander,Svetlana Konovalova,Mügen Terzioglu et al.
Taru Hilander et al.
Mitochondria are multifunctional organelles with their own genome and protein synthesis machinery. The 13 proteins encoded by mitochondrial DNA (mtDNA) are core subunits of the oxidative phosphorylation (OXPHOS) system producing the majorit...
Jacquelynn Benjamino,Lidia Beka,Joerg Graf
Jacquelynn Benjamino
Microbiomes can be thought of as the integration of biotic and abiotic factors, including the microbial communities and the distinct physio-chemical properties that are present in a habitat. The microbes within a microbiome can influence th...
Newly Revised Quantitative PCR-Based Assay for Mitochondrial and Nuclear DNA Damage [0.03%]
一种用于线粒体和核DNA损伤的新定量PCR检测方法
Laurie H Sanders,Jeremy P Rouanet,Evan H Howlett et al.
Laurie H Sanders et al.
Given the crucial role of DNA damage in human health and disease, it is important to be able to accurately measure both mitochondrial and nuclear DNA damage. This article describes a method based on a long-amplicon quantitative PCR-based as...
Accidental and Programmed Cell Death in Investigative and Toxicologic Pathology [0.03%]
病理学和毒理学中的意外及程序性细胞死亡
Zaher A Radi,Zachary S Stewart,Shawn P O&#x;Neil
Zaher A Radi
Cellular development and homeostasis are regulated via programmed cell death (PCD; apoptosis), which is a genetically regulated cellular process. Accidental cell death (ACD; necrosis) can be triggered by chemical, physical, or mechanical st...
Norimasa Tamehiro,Reiko Adachi,Yoshie Kimura et al.
Norimasa Tamehiro et al.
A food allergy is a chronic inflammatory disease against dietary antigens with high prevalence in industrialized countries. Because there is currently no cure for food allergies, avoiding the allergen is crucial for the prevention of an all...