Collection of bile and urine samples for determining the urinary and hepatobiliary disposition of xenobiotics in mice [0.03%]
小鼠外源性物质尿液和肝胆排泄测定的胆汁和尿样采集方法
José E Manautou,Chuan Chen
José E Manautou
One of the most simple and informative method commonly used by researchers in the field of xenobiotics metabolism and disposition to investigate the fate of chemicals in living organisms involves identification and/or quantitation of metabo...
Lisa J Bain
Lisa J Bain
R E Baynes
R E Baynes
Porcine Skin Flow-Through Diffusion Cell System (Ronald E. Baynes, North Carolina State University, Raleigh, North Carolina). Porcine skin can be used in a diffusion cell apparatus to study the rate and extent of absorption of topically app...
R E Baynes
R E Baynes
The isolated porcine skin flap is used to determine the rate and extent of absorption of chemicals through skin. This ex vivo model system allows cutaneous toxicology and pharmacology studies to be conducted in viable skin that has a normal...
M Mayersohn
M Mayersohn
This unit provides an overview of the principles underlying the elimination process. Experimental design issues, methods of data analysis, and complications that may be encountered are discussed. A complete worked example is provided to exe...
Measurement of bioavailability: measuring absorption through skin in vivo in rats and humans [0.03%]
皮肤体外渗透试验的相关性和预测性研究
S V Bounds,D R Hawkins
S V Bounds
Absorption through the skin is a route of exposure to a wide variety of therapeutic and/or environmental compounds. In vivo assays are advantageous in that they retain intact epidermal and dermal structures and thus reflect a more normal si...
Measurement of bioavailability: measurement of absorption through skin in vitro [0.03%]
体外测量皮肤吸收量以测定生物利用度
S A Hotchkiss
S A Hotchkiss
New therapeutic compounds intended for use on the skin or for delivery through application to the skin and agrochemicals, whose use may result in skin exposure, must be tested for bioavailability as the result of absorption. This unit conta...
Arthur J L Cooper,Boris F Krasnikov,John T Pinto et al.
Arthur J L Cooper et al.
Cysteine S-conjugate β-lyases are pyridoxal 5'-phosphate (PLP)-containing enzymes that catalyze the conversion of cysteine S-conjugates [RSCH(2)CH(NH(3) (+))CO(2) (-)] and selenium Se-conjugates [RSeCH(2)CH(NH(3) (+))CO(2) (-)] that contai...
Manipulation of expression of arsenic (+3 oxidation state) methyltransferase in cultured cells [0.03%]
关于砷(III)甲基转移酶培养细胞中表达的调节
Zuzana Drobna,Miroslav Styblo,David J Thomas
Zuzana Drobna
Methylation of inorganic arsenic to produce mono-, di-, or trimethylated products is the central process in the cellular metabolism of arsenic. Identification of arsenic (+3 oxidation state) methyltransferase (As3mt) as the enzyme that coul...
Purification of arsenic (+3 oxidation state) methyltransferase from rat liver cytosol [0.03%]
从大鼠肝胞质中提纯砷(三价)甲基化酶
Zuzana Drobna,Miroslav Styblo,David J Thomas
Zuzana Drobna
Demonstrating the enzymatic basis of arsenic methylation is critical to further studies of the pathway for the conversion of inorganic arsenic into a variety of methylated metabolites. This protocol describes a procedure for the purificatio...