Measurement of the vitamin E metabolites, carboxyethyl hydroxychromans (CEHCs), in biological samples [0.03%]
生物样本中维生素E代谢产物羧乙基羟基查尔酮(CEHC)的测定
Scott W Leonard,Maret G Traber
Scott W Leonard
Metabolites of α- and γ-tocopherol, 2,5,7,8-tetramethyl-2-(2'-carboxyethyl)-6-hydroxychroman (α-CEHC) and 2,7,8-trimethyl-2-(β-carboxyethyl)-6-hydroxychroman (γ-CEHC), respectively, are produced in the liver and have been measured in b...
Catalase activity assays [0.03%]
过氧化氢酶活性测定方法
Nandita Shangari,Peter J O&#x;Brien
Nandita Shangari
Catalase (hydrogen peroxide/hydrogen peroxide oxidoreductase) is an important cellular antioxidant enzyme that defends against oxidative stress. It is found in the peroxisomes of most aerobic cells. It serves to protect the cell from toxic ...
Measurement of ascorbic acid and dehydroascorbic acid in biological samples [0.03%]
生物样品中抗坏血酸和脱氢抗坏血酸的测定
Jens Lykkesfeldt
Jens Lykkesfeldt
Ascorbic acid and dehydroascorbic acid are commonly used biomarkers of oxidative stress in a variety of experimental models. However, the accurate measurement of these labile compounds remains a challenge both in terms of sample collection ...
J M Jacobs,N J Jacobs
J M Jacobs
Protoporphyrinogen oxidase catalyzes the oxidation of protoporphyrinogen to protophyrin. It is a membrane-bound mitochondrial enzyme and it is the target of photobleaching herbicides. The basic assay presented in this unit for measuring oxi...
J D Phillips,J P Kushner
J D Phillips
Uroporphyrinogen decarboxylase (UROD) catalyzes decarboxylation of the four acetate side chains of urophyrinogen to form coproporphyrinogen. Activity of UROD can be measured using an enzymatically prepared substrate or a chemically prepared...
P R Sinclair,N Gorman,J M Jacobs
P R Sinclair
Heme (iron protoporphyrin IX) is a prosthetic group for a number of hemoproteins in different tissues (e.g., hemoglobin, myoglobin, cytochrome P-450s, mitochondrial cytochromes, catalases, and peroxidases). Mutations in the biosynthetic pat...
P R Sinclair,N Gorman,N W Cornell
P R Sinclair
In most cells, δ-aminolevulinate (ALA) synthase is the rate-limiting enzyme in heme synthesis. It is inducible by drugs and toxins and is feedback regulated by heme. This unit describes a radiometric assay using [¹⁴C]succinate as a subst...
The heme biosynthesis pathway and clinical manifestations of abnormal function [0.03%]
血红素生物合成途径及功能异常的临床表现
J D Phillips,J P Kushner
J D Phillips
Biosynthesis of heme is important for both prokaryotes and eukaryotes. The enzymes for this multistep process are distributed between the cytosol and mitochondria in eukaryotes. In humans there are inherited and acquired disorders character...
Measurement of MnSOD and CuZnSOD activity in mammalian tissue homogenates [0.03%]
哺乳动物组织匀浆中MnSOD和CuZnSOD活性的测定
D R Spitz,L W Oberley
D R Spitz
Three basic forms of mammalian SODs exist and they are distinguished by their sizes and locations. SOD enzyme activity is not easily monitored by direct measurement because the substrate disappearance is very rapid at physiological pH. Acti...
E S Arnér,A Holmgren
E S Arnér
The thioredoxin system is ubiquitous, providing reducing equivalents to essential biosynthetic enzymes like ribonucleotide reductase. It is essential for cellular redox regulation, control of oxidative stress, and protection against oxidati...