Dianne M Creasy
Dianne M Creasy
Fixation and sampling of the male reproductive system for routine histopathological examination requires special procedures including the use of nonroutine fixatives and nonroutine stains. High-resolution light microscopy and electron micro...
Leslie B Poole
Leslie B Poole
Protein sulfenic acids are reactive, reversibly oxidized cysteinyl residues with roles in redox catalysis and regulation. Detection and quantification of these species in proteins is accomplished through chemical modification by reagents su...
Barbara D Abbott
Barbara D Abbott
Palatal organ culture provides an in vitro model for the study of the formation of the secondary palate, which forms the roof of the mouth in the developing fetus. The protocol describes the steps for culture of the mid-facial region of the...
Michael G Narotsky,Robert J Kavlock
Michael G Narotsky
This unit presents two basic protocols for testing prenatal developmental toxicity in rodents. The standard protocol for assessing prenatal developmental toxicity, often referred to as a Segment II study, describes the methods used for perf...
Diane S Henshel,Jamie DeWitt,Andrea Troutman
Diane S Henshel
This unit describes methods for injecting, incubating, handling and analyzing domestic chicken embryos used in teratology studies. It also includes a discussion of caveats and special handling issues as well as some discussion of statistica...
R A Ponce
R A Ponce
This unit describes methods for culture of undifferentiated midbrain (mesencephalon) and limb bud cells from gestation day 12 rat embryos. When grown over 5 days in vitro, these mixed cell populations express many morphological, biochemical...
A G Fantel,R Bechter,D Beckman
A G Fantel
Mammalian embryos provide a particular challenge to those who wish to study developmental processes because development takes place inside the mother's body, thus limiting the investigator's ability to directly affect and observe the embryo...
Overview of teratology [0.03%]
胎胚学概论
C A Kimmel
C A Kimmel
Teratology is the study of developmental processes to understand the causes and mechanisms of abnormal developmental outcomes. This unit provides an explanation of the basic principles of the field as a context for the more specialized prot...
Neonatal rat primary microglia: isolation, culturing, and selected applications [0.03%]
新生大鼠原代小胶质细胞的分离、培养及部分应用案例
Mingwei Ni,Michael Aschner
Mingwei Ni
Microglial cells elaborate trophic factors and cytokines and remove toxins and debris from the extracellular space in the central nervous system, acting analogously to peripheral macrophages. Over the past two decades, increased attention h...
Application of single-cell microfluorimetry to neurotoxicology assays [0.03%]
单细胞微荧光测定技术在神经毒理学检测中的应用
Tobi L Limke,William D Atchison
Tobi L Limke
Intracellular signaling events play fundamental roles in regulating physiological function. In neurons, these include inducing growth and differentiation, secretion, gene expression, and controlling processes associated with learning and me...