Michael H Simonian
Michael H Simonian
This unit describes spectrophotometric and colorimetric methods for measuring the concentration of a sample protein in solution. Absorbance measured at 280 nm (A(280)) is used to calculate protein concentration by comparison with a standard...
Sarah M Andrew,Julie A Titus,Louis Zumstein
Sarah M Andrew
Conventional dialysis separates small molecules from large molecules by allowing diffusion of only the small molecules through selectively permeable membranes. Dialysis is usually used to change the salt (small-molecule) composition of a ma...
Sean R Gallagher
Sean R Gallagher
Electrophoresis is used to separate complex mixtures of proteins (e.g., from cells, subcellular fractions, column fractions, or immunoprecipitates), to investigate subunit compositions, and to verify homogeneity of protein samples. It can a...
Mary C Phelan
Mary C Phelan
This appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step-by-step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, f...
M Bergeron,J Honkaniemi,F R Sharp
M Bergeron
This unit describes the processes of extracting RNA from tissues and analyzing it by northern blot hybridization to probe the expression of a particular gene at the transcriptional level.
D Voytas,N Ke
D Voytas
This unit provides procedures for detecting an quantifying radiolabeled proteins in SDS-polyacrylamide gels or blots. Autoradiography requires fixed, dried gels before exposure to X-ray film. The results may be enhanced by using intensifyin...
Enzymatic amplification of DNA by PCR: standard procedures and optimization [0.03%]
DNA聚合酶的体外扩增反应(PCR)标准程序和优化方法研究
M F Kramer,D M Coen
M F Kramer
The polymerase chain reaction (PCR) is used to enzymatically amplify small quantities of specific DNA sequences. The reaction must optimized to specifically amplify the sequences and primers of interest; this includes titration of magnesium...
Molecular biology techniques [0.03%]
分子生物学技术
Protocols presented in Current Protocols in Toxicology sometimes include molecular biological, biochemical, and other biological techniques that may not be fully described in this manual. Although it may be reasonable to assume that readers...
Standard laboratory equipment [0.03%]
标准实验室设备
This appendix lists pieces of equipment that are standard in the modern toxicology laboratory, i.e., items used extensively in this manual and thus not usually included in the individual materials lists. No attempt has been made to list all...