Gunnar F Kwakye,Daphne Li,Olympia A Kabobel et al.
Gunnar F Kwakye et al.
Cellular manganese (Mn) uptake and transport dynamics can be measured using a cellular fura-2 manganese extraction assay (CFMEA). The assay described here uses immortalized murine striatal cell line and primary cortical astrocytes, but the ...
LC-MS/MS quantitation of mercapturic acid conjugates of lipid peroxidation products as markers of oxidative stress [0.03%]
采用氧化应激标志物的硫代酸共轭物定量检测脂质过氧化产物的LC-MS/MS法
Heather C Kuiper,Jan F Stevens
Heather C Kuiper
Oxidative stress-induced lipid peroxidation (LPO) leads to the formation of cytotoxic and genotoxic 2-alkenals. LPO products such as 4-hydroxy-2(E)-nonenal (HNE) and 4-oxo-2(E)-nonenal (ONE) have been the subject of many studies due to thei...
Isolation and applications of prostate side population cells based on dye cycle violet efflux [0.03%]
基于荧光淬灭技术分离并鉴定前列腺侧群细胞及其应用研究
Kalyan J Gangavarpu,Wendy J Huss
Kalyan J Gangavarpu
This unit describes methods for digestion of human prostate clinical specimens and dye cycle violet (DCV) staining for identification, isolation, and quantitation of radiolabeled dihydrotestosterone (DHT) retention of side population cells....
Using chemotherapeutic drugs to trigger hematopoietic stem cell activation and determine hematological toxicity [0.03%]
利用化疗药物激活造血干细胞并判断血液毒性大小
Nathan Boles
Nathan Boles
Hematopoietic stem cells (HSCs) replenish blood cells throughout the lifetime of an animal. Maintaining the HSC pool is of paramount importance to the survival of the organism. Thus, when treating a patient or an animal with chemotherapeuti...
Stem cells in toxicology [0.03%]
毒理学中的干细胞
David Lawrence
David Lawrence
Isabella De Angelis,Laura Turco
Isabella De Angelis
The Caco-2 cell system, a well characterized intestinal in vitro model, makes it possible to evaluate the ability of chemicals to cross the intestinal barrier, as well as to study their transport mechanisms. Permeability values estimated wi...
Brad L Upham
Brad L Upham
Gap junctional intercellular communication (GJIC) plays a central role in coordinating signal-transduction pathways that control gene expression inside of cells with those of neighboring cells in maintaining the homeostasis of a tissue. The...
Assessment of gastrointestinal propulsive activity using three different models of peristalsis in vivo in the mouse [0.03%]
基于小鼠在体三种不同蠕动模型的胃肠道推进功能评价研究
Enzo Poli,Cristina Pozzoli
Enzo Poli
The protocols described in this unit are designed to assess the acute effects of drugs on the propulsive activity of the gastrointestinal muscles in the conscious mouse. These protocols are currently applied to investigate the pharmacologic...
Assessment of gastrointestinal motility using three different assays in vitro [0.03%]
体外三种不同的测定方法评估胃肠道运动功能
Cristina Pozzoli,Enzo Poli
Cristina Pozzoli
The protocols detailed in this unit are designed to assess the motor activity of different gastric and intestinal muscle preparations in vitro and the effects of drugs that modulate gastrointestinal motility. The preparations described are ...
Randall I Krohn
Randall I Krohn
Protein quantification is an important step for handling protein samples for isolation and characterization; it is a prerequisite step before submitting proteins for chromatographic, electrophoretic, or immunochemical analysis and separatio...