Preparation of epithelial and mesenchymal stem cells from murine mammary gland [0.03%]
从小鼠乳腺中制备上皮干细胞和间充质干细胞
Ian Guest,Zoran Ilic,Jun Ma
Ian Guest
The mammary gland is a complex organ consisting of multiple cell types that undergo extensive remodeling during pregnancy and involution, cyclical changes that suggest the existence of a resident stem cell population that is responsible for...
Murine embryonic stem cell derivation, in vitro pluripotency characterization, and in vivo teratoma formation [0.03%]
小鼠胚胎干细胞的建系、体外多能性鉴定和体内致畸性试验
Yu-Fen Chou,Akiko Yabuuchi
Yu-Fen Chou
The derivation of embryonic stem (ES) cells represents one of the most important breakthroughs in mammalian developmental biology. In addition to their utility in a wide array of in vitro studies, ES cells are also one of the most useful st...
Florianne Monnet-Tschudi,Antoinette Defaux,Olivier Braissant et al.
Florianne Monnet-Tschudi et al.
Neuroinflammation is observed in many brain pathologies: in neurodegenerative diseases and multiple sclerosis as well as in chemically induced lesions. It is characterized by the reactivity of microglial cells and astrocytes, activation of ...
Overview of peroxiredoxins in oxidant defense and redox regulation [0.03%]
过氧化氢酶茋在抗氧化防御和氧化还原调节中的作用概述
Leslie B Poole,Andrea Hall,Kimberly J Nelson
Leslie B Poole
Peroxiredoxins are important hydroperoxide detoxification enzymes, yet have only come to the fore in recent years relative to the other major players in peroxide detoxification, heme-containing catalases and peroxidases and glutathione pero...
Kimberly J Nelson,Derek Parsonage
Kimberly J Nelson
Peroxiredoxins are cysteine-dependent peroxidases that react with hydrogen peroxide, larger hydroperoxide substrates, and peroxynitrite. Protocols are provided to measure Prx activity with peroxide by (1) a coupled reaction with NADPH, thio...
Linda L Agnew
Linda L Agnew
Heat shock or stress proteins are constitutively expressed redox-sensitive proteins, the synthesis of which is induced in almost all organisms exposed to a range of stressors, including heat shock, oxidative stress, free radicals, UV radiat...
Assessment of mitochondrial toxicity in HepG2 cells cultured in high-glucose- or galactose-containing media [0.03%]
基于高糖或半乳糖培养基的肝癌细胞株HepG2线粒体毒性的评价研究
Rachel Swiss,Yvonne Will
Rachel Swiss
Drug-induced mitochondrial toxicity has been recognized as contributing to a variety of organ toxicities, such as liver, heart, kidney, and CNS, and has been found to contribute to drug attrition and black box warnings. Here, we describe a ...
Immaculate Amunom,Sanjay Srivastava,Russell A Prough
Immaculate Amunom
This protocol describes the procedure for measuring the relative rates of metabolism of the α,β-unsaturated aldehydes 9-anthracene aldehyde (9-AA) and 4-hydroxy-trans-2-nonenal (4-HNE). More specifically, these assays measure the aldehyde...
High-throughput assays for assessing mitochondrial dysfunction caused by compounds that impair mtDNA-encoded protein levels in eukaryotic cells [0.03%]
用于评估化合物损害真核细胞线粒体DNA编码蛋白水平所致线粒体功能障碍的高通量检测方法
Sashi Nadanaciva,James Murray,Casey Wilson et al.
Sashi Nadanaciva et al.
Compounds that impair the synthesis of either mitochondrial DNA (mtNDA) or mtDNA-encoded proteins reduce the levels of 13 proteins essential for oxidative phosphorylation, leading to a decrease in mitochondrial ATP production. Toxicity caus...
Fluorescence correlation spectroscopy: detecting and interpreting the mobility of transmembrane proteins in vivo [0.03%]
荧光相关光谱法：检测并解释活细胞中跨膜蛋白的运动性
Nina Malchus
Nina Malchus
Mobility of proteins is crucial for their functionality. Fluorescence correlation spectroscopy (FCS) is a sensitive tool for assessing dynamics in vivo. It can reveal properties of diffusing proteins, as well as of the surrounding medium. H...