Richard Cole
Richard Cole
Light microscopy has played a central role in science for the past couple of hundred years and will continue to do so. Multiple super-resolution microscopy techniques have been in the headlines for smashing what for more than 100+ years was...
Generation of Macrophages from Cynomolgus-Monkey Bone Marrow as a Model to Evaluate Effects of Drugs on Innate Immunity [0.03%]
利用食蟹猴骨髓生成巨噬细胞作为评价药物对固有免疫影响的模型
Nianyu Li,Susan A Ludmann,Lisa Anest et al.
Nianyu Li et al.
Macrophages are innate immune cells that play important roles in various physiological and pathological processes. Evaluation of pro-inflammatory effects of drugs on macrophages has become commonplace in preclinical drug development prior t...
Analysis of Human Mitochondrial DNA Content by Southern Blotting and Nonradioactive Probe Hybridization [0.03%]
人类线粒体DNA含量的Southern blot分析及非放射性探针杂交方法
Joel H Wheeler,Carolyn K J Young,Matthew J Young
Joel H Wheeler
A single cell can contain several thousand copies of the mitochondrial DNA genome or mtDNA. Tools for assessing mtDNA content are necessary for clinical and toxicological research, as mtDNA depletion is linked to genetic disease and drug to...
Mitochondrial Electron Transfer Cascade Enzyme Activity Assessment in Cultured Neurons and Select Brain Regions [0.03%]
培养神经元和选定脑区的线粒体电子传递链酶活性评估
Abdulbaki Agbas,Partha Krishnamurthy,Mary L Michaelis et al.
Abdulbaki Agbas et al.
Measurement of the electron transfer cascade (ETC) enzyme activities and their kinetic profiles is important in assessing mitochondrial function in the nervous system in health and disease or following exposure to toxic agents. The optimiza...
The Use of Intracerebral Microdialysis to Elucidate Environmentally Induced Neurotoxic Mechanisms [0.03%]
利用脑内微透析技术阐明环境因素诱导的神经毒性机制
Stephen M Lasley
Stephen M Lasley
The technique of microdialysis permits the assessment of neurotransmitter activity and the monitoring of other cellular entities in tissue extracellular fluid. The method is widely used for quantifying biogenic amine and amino acid transmit...
Diane Frances Lee,Mark Andrew Chambers
Diane Frances Lee
Alveolar type II (ATII) cells play a key role as part of the distal lung epithelium, including in the innate immune response and as self-renewing progenitors to replace alveolar type I (ATI) cells during epithelial regeneration. Their secre...
Preparation and Measuring of Brain GABAA R-coupled Cl- /HCO3- - Activity for Integral Assessment of Aquatic Toxicity [0.03%]
脑GABAaR偶联Cl-/HCO3-活性的准备和测定用于全面评价水体毒性的影响因素及作用机制研究
Sergey A Menzikov
Sergey A Menzikov
The wide use of aromatic hydrocarbons in various industries is having a negative effect on the environment and human health. Therefore, a key focus of current toxicology is the development and use of protein reporters with high sensitivity ...
Inducing a Mucosal Barrier-Sparing Inflammatory Response in Laboratory-Grown Primary Human Nasal Epithelial Cells [0.03%]
在实验室培养的原代人类鼻上皮细胞中诱导粘膜屏障保护型炎性反应
Mahnaz Ramezanpour,Harrison Bolt,Alkis Psaltis et al.
Mahnaz Ramezanpour et al.
Here we use the toll-like receptor (TLR) 3 agonist poly I:C (LMW) to induce an inflammatory response in cells of submerged and/or air-liquid interface (ALI) cultures of human nasal epithelial cells (HNECs). The inflammatory response is dete...
In Vitro Monocyte/Macrophage Phagocytosis Assay for the Prediction of Drug-Induced Thrombocytopenia [0.03%]
体外单核细胞/巨噬细胞吞噬试验在预测药物所致血小板减少中的应用
Padma Kumar Narayanan,Nianyu Li
Padma Kumar Narayanan
Phagocytosis of platelets by monocytes and macrophages is a primary mechanism of platelet clearance in vivo and has been increasingly implicated in playing an important role in thrombocytopenia mediated by monoclonal antibodies intended for...
Jenifer A Bradley,Christopher J Strock
Jenifer A Bradley
Neurotoxicity and seizurogenic liabilities are difficult to detect using currently available in vitro cytotoxicity assays. This is primarily due to the inherent limitations of these assays to predict adverse neural network disruptions and c...