Kamala Pant,Marilyn J Aardema
Kamala Pant
The Syrian hamster embryonic (SHE) cell transformation assay (CTA) at pH 6.7 can determine the ability of a test article to induce morphological transformation (MT) in cultured SHE cells. The assay uses SHE cells prepared at gestation day ...
Courtney E W Sulentic
Courtney E W Sulentic
Immunoglobulins (Ig) are critical in maintaining host immunity to a variety of pathogens. Regulation of Ig expression is a complex process involving transcriptional regulation of different Ig gene loci by many transcription factors and tran...
Identification and characterization of oxylipid-protein and peptide conjugates by mass spectrometry [0.03%]
利用质谱法鉴定和表征氧化脂-蛋白质及肽类加合物
Woon-Gye Chung,Claudia S Maier
Woon-Gye Chung
The modification of proteins by reactive products of lipid peroxidation is associated with a large number of diseases and biological aging; thus, methods that enable the characterization of oxylipid-protein and/or peptide conjugates are hig...
Latha Diwakar,Ajit Ray,Vijayalakshmi Ravindranath
Latha Diwakar
Mammalian NADH:ubiquinone oxidoreductase (complex I) is made up of at least 46 subunits and is one of the largest enzyme complexes known. It catalyzes the first step of the respiratory electron transport chain through the oxidation of NADH,...
Swapna V Shenvi,Brian M Dixon,Kate Petersen Shay et al.
Swapna V Shenvi et al.
The purpose of this protocol is to establish a primary hepatocyte culture system as a suitable model to examine age-related changes in Phase II detoxication gene expression. Hepatocytes are isolated using a two-step collagenase perfusion te...
Experimental models of hypoxic-ischemic encephalopathy: hypoxia-ischemia in the immature rat [0.03%]
低氧缺血性脑病的实验模型：幼年大鼠的低氧缺血损伤
Silvia Carloni,Walter Balduini
Silvia Carloni
The development of experimental models to study the mechanisms of perinatal hypoxic-ischemic encephalopathy and stroke and effective therapies represents an important goal in perinatal medicine. However, due to the complexity of this pathol...
Renal transporters in drug disposition, drug-drug interactions, and nephrotoxicity [0.03%]
药物代谢动力学、药物相互作用和肾毒性的肾转运体
Bo Feng,Ayman F El-Kattan,Zaher A Radi
Bo Feng
This unit describes in detail the in vitro methods for measuring the interaction of new chemical entities (NCEs) with human renal transporters (hOAT1, hOAT2, and hOCT2) as both a substrate and inhibitor. Renal transporter substrate assays h...
Evaluation of gastric ulcerogenic and healing impairment effects of bisphosphonates: adverse gastric reactions of bisphosphonate [0.03%]
双膦酸盐胃溃疡诱导和愈合抑制作用的评价及不良胃反应
Koji Takeuchi,Kikuko Amagase
Koji Takeuchi
Bisphosphonates (BPPs) were developed as antiresorptive drugs capable of treating diseases related to bone remodeling; however, they have untoward effects including ulceration in the upper gastrointestinal tract and worsen the healing-impai...
Mining the salivary proteome with grating-coupled surface plasmon resonance imaging and surface plasmon coupled emission microarrays [0.03%]
应用光栅耦合表面等离子体共振成像和表面等离子体偶联发射微阵列技术挖掘唾液蛋白质组学
Ryan D Molony,James M Rice,Jong Seol Yuk et al.
Ryan D Molony et al.
Biological indicators have numerous and widespread utility in personalized medicine, but the measurement of these indicators also poses many technological and practical challenges. Blood/plasma has typically been used as the sample source w...
Assessing cell fusion and cytokinesis failure as mechanisms of clone 9 hepatocyte multinucleation in vitro [0.03%]
体外评估克隆9肝细胞多核形成的细胞融合和分裂末期失败机制
Damir Simic,Catherine Euler,Christina Thurby et al.
Damir Simic et al.
In this in vitro model of hepatocyte multinucleation, separate cultures of rat Clone 9 cells are labeled with either red or green cell tracker dyes (Red Cell Tracker CMPTX or Vybrant CFDA SE Cell Tracer), plated together in mixed-color colo...