Analysis of Protein-Protein Interactions by Split Luciferase Complementation Assay [0.03%]
利用分裂萤光素酶互补技术分析蛋白质相互作用
Yuekun Lang,Zhong Li,Hongmin Li
Yuekun Lang
Protein-protein interactions are important in human disease. Developing and refining tools to understand physical contacts between signaling proteins is crucial. This article describes a split luciferase complementation (SLC) method designe...
Culturing Rat Whole Ovary for UV Filter Benzophenone-3 Treatment [0.03%]
用于紫外线吸收剂苯甲酮-3培养的完整大鼠卵巢体外处理方法
Clarisa Guillermina Santamaría,Julián Elías Abud,Enrique Hugo Luque et al.
Clarisa Guillermina Santamaría et al.
We describe a detailed protocol to establish a newborn rat whole ovary culture, which enables the study of direct effects (independent of hypothalamic-pituitary-gonadal axis) of endocrine disrupting chemicals (EDCs), such as benzophenone-3 ...
Bianca Marigliani,Luciene Bottentuit López Balottin,Elisabeth de Fatima Pires Augusto
Bianca Marigliani
In order to circumvent ethical, technical, and economic drawbacks regarding the use of animal serum in cell culturing, it is possible to adapt mammalian cells to serum-free media. Nowadays, there are several serum-free formulations availabl...
Generation and Validation of Tissue-Specific Knockout Strains for Toxicology Research [0.03%]
用于毒理学研究的组织特异性基因敲除小鼠品系的制备及验证
Cherish A Taylor,William Shawlot,Jin Xiang Ren et al.
Cherish A Taylor et al.
Tissue-specific knockout mice are widely used throughout scientific research. A principle method for generating tissue-specific knockout mice is the Cre-loxP system. Here, we give a detailed description of the steps required to generate and...
Preparation of Primary Rat Hepatocyte Spheroids Utilizing the Liquid-Overlay Technique [0.03%]
利用液体覆盖技术制备大鼠原代肝细胞球体
Jonathan A Kyffin,Christopher R Cox,Joseph Leedale et al.
Jonathan A Kyffin et al.
Herein, we describe a protocol for the preparation and analysis of primary isolated rat hepatocytes in a 3D cell culture format described as spheroids. The hepatocyte cells spontaneously self-aggregate into spheroids without the need for sy...
Tina B McKay,Andrew Ford,Siran Wang et al.
Tina B McKay et al.
The cornea provides a functional barrier separating the outside environment from the intraocular environment, thereby protecting posterior segments of the eye from infection and damage. Pathological changes that compromise the structure or ...
Assessment of Sertoli Cell Proliferation by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide and Sulforhodamine B Assays [0.03%]
3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑和磺基罗丹明B染色法检测生精细胞的增殖能力
Ana D Martins,Pedro F Oliveira,Marco G Alves
Ana D Martins
The correct functioning of Sertoli cells (SCs) is pivotal for successful spermatogenesis. They are major targets for hormones, endocrine disruptors, and other substances that men are subjected to every day. One of the main SC functions that...
Anjaney Kothari,Padmavathy Rajagopalan
Anjaney Kothari
The small intestine is an important organ primarily involved in digestion of food and absorption of nutrients. In vitro intestinal models are being developed to study this organ in health and disease. Intestinal explants can be used in such...
A Reliable Preclinical Model to Study the Impact of Cigarette Smoke in Development and Disease [0.03%]
一种可靠的前期模型来研究香烟烟雾对发育和疾病的影响
Geraldine Aedo,Miguel Miranda,Myra N Chávez et al.
Geraldine Aedo et al.
The World Health Organization has estimated that, worldwide, cigarette smoking has caused more than 100 million deaths in the last century, a number that is expected to increase in the future. Understanding cigarette smoke toxicity is key f...
Acute Metabolic Switch Assay Using Glucose/Galactose Medium in HepaRG Cells to Detect Mitochondrial Toxicity [0.03%]
基于葡糖糖/半乳糖培养基的急性代谢转换试验在肝细胞系中的应用以筛查线粒体毒性
Laleh Kamalian,Oisin Douglas,Carol E Jolly et al.
Laleh Kamalian et al.
Using galactose instead of glucose in the culture medium of hepatoma cell lines, such as HepG2 cells, has been utilized for a decade to unmask the mitochondrial liability of chemical compounds. A modified glucose-galactose assay on HepG2 ce...