Igor Branchi,Daniela Santucci,Enrico Alleva
Igor Branchi
Altricial rodent pups emit ultrasonic vocalizations (USVs), which are whistle-like sounds with frequencies between 30 and 90 kHz. These signals play an important communicative role in mother-offspring interaction because they elicit in the ...
Igor Branchi,Laura Ricceri
Igor Branchi
Learning and memory abilities can be reliably measured in rodents starting from very early phases of postnatal development. In particular, in the study of learning and memory in periweanling or adolescent (from around postnatal day 20 to 50...
Lakshmaiah Sreerama,Norman E Sládek
Lakshmaiah Sreerama
Seventeen enzymes are currently viewed as belonging to the human aldehyde dehydrogenase superfamily, and all of them catalyze the pyridine nucleotide-dependent oxidation of aldehydes to acids. Depending on the specific aldehyde dehydrogenas...
M Jane Cox Rosemond
M Jane Cox Rosemond
Carbonyl reducing enzymes are involved in the metabolism of endogenous as well as xenobiotic molecules. Enzymes that catalyze the reversible oxidoreduction of aldehyde and ketone moieties include alcohol dehydrogenases, aldo-keto reductases...
Lauren A Trepanier,Sunil U Bajad,Joseph R Kurian
Lauren A Trepanier
NADH cytochrome b(5) reductase (b(5)R; EC 1.6.2.2; Diaphorase I; NADH: ferricytochrome b(5) oxidoreductase) is an FAD-containing protein, which, along with the hemoprotein cytochrome b(5) (cyt b(5)), mediates electron transfer from NADH to ...
TaqMan real time-polymerase chain reaction methods for determination of nucleotide polymorphisms in human N-acetyltransferase-1 (NAT1) and -2 (NAT2) [0.03%]
TaqMan 实时聚合酶链反应测定人类乙酰转移酶-1(NAT1)和-2(NAT2)核苷酸多态性的方法
David W Hein,Mark A Doll
David W Hein
N-acetyltransferase 1 (NAT1) and N-acetyltransferase 2 (NAT2) exhibit allelic variation and genetic polymorphism associated with increased susceptibility towards drug toxicity and environmental disease. TaqMan allelic discrimination methods...
In vitro model for modulation of helper T cell differentiation and activation [0.03%]
体外辅助性T细胞分化及活化的调控模型
Yong Heo
Yong Heo
Influence of immunotoxicants on helper T cell reactivities can be investigated by assessing acquisition of IL-4- or IFN-γ-producing ability in vitro with antigen-primed naive or precursor helper T cells from the spleens of DO11.10 ovalbumi...
Julie A Oughton,Nancy I Kerkvliet
Julie A Oughton
Immunophenotyping of lymphoid tissues, particularly spleen and thymus, has been used in the field of immunotoxicology for many years as part of a tiered screening approach for detecting immunotoxicity following chemical exposure. This unit ...
Solid-phase immunoassays [0.03%]
固相免疫测定法
Michael A Lynes
Michael A Lynes
Solid-phase quantitative immunoassays are some of the most commonly used diagnostic tests for both soluble antigen composition and the assessment of cellular functions. These immunoassays (e.g., ELISA, ELISPOT) provide a highly sensitive me...
B Paige Lawrence
B Paige Lawrence
The protocols in this unit describe how to measure the activity of the two most common types of lymphocytes that have cytolytic activity: cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Measurement of CTL activity requires prim...