Aleksandra Filipovska,Michael P Murphy
Aleksandra Filipovska
Proteins contain free, exposed thiols that can be glutathionylated in the native state as a result of thiol-disulfide exchange reactions with glutathione disulfide, catalyzed by glutaredoxin. A number of other reactions can also lead to pro...
Aleksandra Filipovska,Michael P Murphy
Aleksandra Filipovska
Many proteins contain free thiols that can be modified by the reversible formation of mixed disulfides with glutathione. Protein glutathionylation is of significance for defense against oxidative damage and in redox signaling. Here we outli...
Monoamine oxidase assays [0.03%]
单胺氧化酶测定法
Keith F Tipton,Gavin Davey,Martha Motherway
Keith F Tipton
Monoamine oxidase catalyzes the oxidative deamination of primary aliphatic and aromatic amines and some secondary and tertiary amines, including the hormone and neurotransmitter amines epinephrine, dopamine, norepinephrine and serotonin. Th...
NMR analysis of thiodiglycol oxidation by Mammalian alcohol dehydrogenases [0.03%]
哺乳动物醇脱氢酶催化的硫代二甘醇的氧化反应的核磁分析研究
Mark J Novak,Alan A Brimfield
Mark J Novak
Nuclear magnetic resonance spectroscopy (NMR) is a powerful technique for elucidating the metabolism of xenobiotics, as it allows for the least ambiguous assignment of chemical structure when compared to other forms of spectroscopy. In addi...
Measurement of human cytochrome P4501A2 (CYP1A2) activity in vitro [0.03%]
人细胞色素P450 1A2(CYP1A2)的体外活性测定法研究
Thomas M Polasek,David J Elliot,John O Miners
Thomas M Polasek
Cytochrome P4501A2 (CYP1A2) is responsible for the metabolism of a diverse range of clinically used drugs and dietary and environmental chemicals (including many procarcinogens). CYP1A2 expression is influenced by numerous factors, and henc...
Huixian Wang,John B Hays
Huixian Wang
This unit describes an assay for completed mismatch correction along with a precise and sensitive assay for mismatch-repair excision intermediates and a simple and efficient procedure for construction of mismatch repair substrates. These su...
Stefan Nierkens,Raymond Pieters
Stefan Nierkens
Many chemicals, including drugs and environmental pollutants, may have the intrinsic capacity to stimulate or dysregulate immune responses. These responses may create considerable problems for exposed subjects in terms of development of aut...
Analysis of immunotoxicity by enumeration of antibody-producing B cells [0.03%]
通过计数抗体产生B细胞进行免疫毒理学分析
Stacey E Anderson,Albert E Munson,Barbara J Meade
Stacey E Anderson
The plaque-forming cell (PFC) assay measures the humoral immune response mediated by the concerted actions of antigen-presenting cells, T lymphocytes, and B lymphocytes. The most common form of the plaque method is used for the detection of...
Ling Cao
Ling Cao
This unit describes a model for the determination of host resistance in mice using a Gram-positive intracellular bacterium, Listeria monocytogenes (LM), and discusses its broad use in immunotoxicological studies. The provides detailed proce...
Linda L Agnew,Kenneth Watson
Linda L Agnew
Heat shock or stress proteins are constitutively expressed redox-sensitive proteins, the synthesis of which is induced in almost all organisms exposed to a range of stressors, including oxidative stress, heat shock, free radicals, UV radiat...