In vitro evaluation of metabolic drug-drug interactions: a descriptive and critical commentary [0.03%]
体外药物代谢相互作用评价：描述和评论
Albert P Li
Albert P Li
Adverse drug-drug interactions represent a major challenge for the pharmaceutical industry. Recently, in vitro approaches for the evaluation of metabolism-related drug-drug interactions have been developed. These in vitro approaches are fou...
Matthew K Ross,Abdolsamad Borazjani
Matthew K Ross
The carboxylesterases (CEs) are hydrolytic enzymes that metabolize xenobiotics that contain ester, thioester, or amide bonds. CEs are ubiquitously expressed but are found in highest concentration in membrane-enriched fractions of the liver....
Christophe Morisseau,Bruce D Hammock
Christophe Morisseau
The human soluble epoxide hydrolase (sEH; EC 3.3.3.2) is the product of the EXPH2 gene. The sEH catalyzes the addition of a water molecule to an epoxide, resulting in the corresponding diol. Early work suggested a role of sEH in detoxifying...
Biochemical and genetic analysis of NAD(P)H:quinone oxidoreductase 1 (NQO1) [0.03%]
NAD(P)H：喹啉氧化还原酶1（NQO1）的生化和基因分析
David Siegel,Jadwiga K Kepa,David Ross
David Siegel
NAD(P)H:quinone oxidoreductase 1 (NQO1, DT-diaphorase, E.C. 1.6.99.2) is an FAD containing obligate two-electron reductase that catalyzes the reduction of a broad range of substrates. This unit will describe methods for the detection of NQO...
Augusto Pessina,Arianna Bonomi
Augusto Pessina
To study hematotoxicity of compounds on the myeloid cell compartment, the authors describe a standard procedure developed as a workable good laboratory practices-compliant protocol to determine the in vitro myelotoxic effect of drugs and ch...
James S Bus
James S Bus
The modern era of chemistry has resulted in dramatic improvements in the overall human condition. In addition to new pharmaceuticals, modern chemistry has brought with it enormous health and social benefits Introduction of chemistry-based t...
Michael A Dorato,Lorrene A Buckley
Michael A Dorato
The primary objective of toxicology studies in the drug discovery process is to evaluate the safety of potential drug candidates. This is accomplished using relevant animal models and validated procedures. The ultimate goal is to translate ...
Fluorescence microscopy and flow cytometric analysis of Peyer's patches and intestinal immune cells [0.03%]
皮尔杰氏垫和肠道免疫细胞的荧光显微镜及流式分析法研究
María C López
María C López
In recent years researchers have become more aware of the importance of the gut-associated immune system since it is in direct interaction with the entry site for virus, bacteria, and all type of food contaminants, including numerous toxins...
Sachin S Devi,Prajakta S Palkar,Harihara M Mehendale
Sachin S Devi
Many hepatotoxicants like acetaminophen, chloroform, carbon tetrachloride, halothane, and thioacetamide cause hepatotoxicity through covalent binding of their reactive metabolites to proteins. The covalent binding to proteins may lead to dy...
Barry W Wilson,John D Henderson
Barry W Wilson
The widespread use of organophosphate and organocarbamate pesticides and the dangers of related chemical warfare agents dramatize the importance of rapid, accurate, and sensitive assays for blood and tissue cholinesterases (ChEs), important...