John B Morris,Joseph A Cichocki,Gregory J Smith
John B Morris
The response of respiratory tract tissue target sites to inhaled vapors depends on the amount of inhaled vapor that is delivered to those sites. Direct measurement of vapor absorption within a specific airway of the living animal requires t...
High-throughput, cell-based screens to identify small-molecule inhibitors of ricin toxin and related category b ribosome inactivating proteins (RIPs) [0.03%]
用于识别蓖麻毒素及相关类别B蛋白的高通量细胞基抑制剂筛选试验
Paul G Wahome,Nicholas J Mantis
Paul G Wahome
Ricin is a member of the ubiquitous ribosome-inactivating protein (RIP) family of toxins. The Centers for Disease Control and Prevention (CDC) classify ricin and related toxins as Category B biothreat agents. There are currently no antidote...
Susanna Wegner,Sungwoo Hong,Xiaozhong Yu et al.
Susanna Wegner et al.
Male reproductive development is a complex process that is sensitive to disruption by a range of toxicants. There is a great need for in vitro models that can evaluate potential male reproductive toxicants. The current unit presents a proto...
High-throughput gene silencing and mRNA expression analysis in hepatocyte sandwich cultures [0.03%]
高通量基因沉默和mRNA表达分析在肝细胞夹心培养中的应用
Brett D Hollingshead,Lauren M Gauthier,Andrew D Burdick
Brett D Hollingshead
Primary hepatocyte sandwich cultures are useful for a variety of research applications where maintenance of metabolic competency is essential. To ensure an optimal hepatocellular phenotype, cells are seeded on collagen-coated dishes and emb...
Carlo J van Staden,Ryan E Morgan,Bharath Ramachandran et al.
Carlo J van Staden et al.
The use of plasma membrane vesicles that overexpress the bile salt export pump (BSEP) or multidrug resistance-associated protein 2, 3, or 4 (MRP2-4) with an in vitro vacuum filtration system offers a rapid and reliable means for screening d...
The vesicular transport assay: validated in vitro methods to study drug-mediated inhibition of canalicular efflux transporters ABCB11/BSEP and ABCC2/MRP2 [0.03%]
囊泡转运检测法：用于研究药物介导的肝胆管外排转运体ABCB11/ BSEP和ABCC2/ MRP2抑制作用的体外实验方法学验证
Krisztina Herédi-Szabó,Emese Kis,Peter Krajcsi
Krisztina Herédi-Szabó
The canalicular membrane of hepatocytes contains several transport proteins that use the energy of ATP to efflux potentially toxic molecules to the bile. Probably the two most important proteins at this location are MRP2 and BSEP, which tra...
Cristina Pozzoli,Enzo Poli
Cristina Pozzoli
The protocol detailed in this unit is designed to assess intestinal peristaltic motility in the isolated small intestine in vitro and to measure the effects of drugs able to interfere with gut propulsive activity. The procedure is based on ...
The NCTC 2544 IL-18 assay for the in vitro identification of contact allergens [0.03%]
NCTC 2544 IL-18试验在体外鉴定接触致敏物中的应用
Valentina Galbiati,Emanuela Corsini
Valentina Galbiati
Assessment of allergenic potency of low-molecular-weight compounds is generally performed using animal models, such as the murine Local Lymph Node Assay (LLNA) and the Guinea Pig Maximization Test (GMT). Progress in understanding the mechan...
Lawrence H Lash
Lawrence H Lash
The cysteine conjugate β-lyase represents activities in cytoplasm and mitochondria catalyzed by at least eleven pyridoxal 5'-phosphate (PLP)-dependent enzymes in various tissues. These enzymes mediate bioactivation of cysteine S-conjugates...
Lawrence H Lash
Lawrence H Lash
The classical view of the glutathione (GSH) conjugation pathway involves GSH S-transferase (GST)-dependent formation of thioether conjugates between GSH and an electrophilic substrate, processing to yield the corresponding cysteine S-conjug...