Matthew D Rand,Sara L Montgomery,Lisa Prince et al.
Matthew D Rand et al.
The fruit fly (Drosophila melanogaster) has long been a premier model for developmental biologists and geneticists. In toxicology studies, Drosophila has only recently gained broader recognition as a tool to elaborate molecular genetic mech...
Kristin M Bircsak,Christopher J Gibson,Robert W Robey et al.
Kristin M Bircsak et al.
ATP-binding cassette (ABC) proteins, including the breast cancer resistance protein (BCRP) and multidrug resistance proteins (MDRs), actively transport structurally diverse chemicals from a number of tissues and are being increasingly cited...
Multi-parametric analysis of cell death pathways using live-cell microscopy [0.03%]
基于活细胞显微镜的细胞死亡途径的多参数分析方法研究
Gaurav N Joshi,David A Knecht
Gaurav N Joshi
Programmed cell death is a complex process with new forms being discovered with regularity. Each pathway has a distinct and overlapping biochemical and physiological change occurring as the cell prepares for death. Detection of these change...
In vitro assays of chemotaxis as a window into mechanisms of toxicant-induced immunomodulation [0.03%]
体外化学趋向性实验作为有毒物质诱导免疫调节机制的窗口
Kathryn M Pietrosimone,Sadikshya Bhandari,Michael G Lemieux et al.
Kathryn M Pietrosimone et al.
Dysregulated cell movement can lead to developmental abnormalities, neoplasia, and immune system disorders, and there are a variety of contexts in which xenobiotics (and biologic) effects on this movement are of interest. Many toxins and to...
Measurement of arginine metabolites: regulators of nitric oxide metabolism [0.03%]
精氨酸代谢产物：一氧化氮代谢的调节物测量
Molly S Augustine,Lynette K Rogers
Molly S Augustine
Arginine is the substrate for nitric oxide synthases (NOS), and arginine availability regulates the production of nitric oxide. Through the activity of methyltransferases, arginine can be methylated to form monomethylarginine (NMMA), asymme...
Robyn L Poerschke,Kristofer S Fritz,Christopher C Franklin
Robyn L Poerschke
Glutathionylation is a posttranslational modification that results in the formation of a mixed disulfide between glutathione and the thiol group of a protein cysteine residue. Glutathionylation of proteins occurs via both nonenzymatic mecha...
In vivo measurement of intragastric pressure with a rubber balloon in the anesthetized rat [0.03%]
麻醉大鼠胃内压力的测定
Zoltán S Zádori,Klára Gyires
Zoltán S Zádori
The protocols described in this unit are designed to measure the intragastric pressure in anesthetized rats by a water-filled low-compliance rubber balloon. The balloon is introduced into the stomach either orally (by passing the balloon do...
Simultaneous, untargeted metabolic profiling of polar and nonpolar metabolites by LC-Q-TOF mass spectrometry [0.03%]
LC-Q-TOF质谱同时非靶向分析极性和非极性代谢物的代谢轮廓
Jay S Kirkwood,Claudia Maier,Jan F Stevens
Jay S Kirkwood
At its most ambitious, untargeted metabolomics aims to characterize and quantify all of the metabolites in a given system. Metabolites are often present at a broad range of concentrations and possess diverse physical properties complicating...
Yung-Wei Pan,Wenbin Wang,Zhengui Xia
Yung-Wei Pan
Adult neurogenesis is a lifelong developmental process that occurs in two discrete regions in the adult mammalian brain: the subgranular zone of the dentate gyrus (DG) and the subventricular zone (SVZ) along the lateral ventricles. Despite ...
The use of fish-derived cell lines for investigation of environmental contaminants: an update following OECD's fish toxicity testing framework No. 171 [0.03%]
鱼类细胞系在环境污染物研究中的应用及经济合作与发展组织第171号鱼类毒性测试框架之后的更新
Vivian R Dayeh,Niels C Bols,Katrin Tanneberger et al.
Vivian R Dayeh et al.
Protocols for evaluating chemical toxicity at the cellular level using fish cell lines are described in this unit. Routine methodologies for growing salmonid cell lines, and using them in aquatic toxicology studies that support the mandate ...