F3-Isoprostanes as a Measure of in vivo Oxidative Damage in Caenorhabditis elegans [0.03%]
模式生物秀丽杆线虫（Caenorhabditis elegans）体内氧化损伤的检测指标F3-异前列烷（F3-isoP）的研究
Thuy T Nguyen,Michael Aschner
Thuy T Nguyen
Oxidative stress has been implicated in the development of a wide variety of disease processes, including cardiovascular disease, cancer, and neurodegenerative diseases, as well as progressive and normal aging processes. Isoprostanes (IsoPs...
Gaoyuan Cao,Valsamma Abraham,Horace M DeLisser
Gaoyuan Cao
The isolation of endothelial cells (ECs) from knockout and transgenic mouse lines provides the opportunity to study the endothelial-specific activities of a targeted molecule. As a means of pursuing these types of investigations, the protoc...
Current Concepts in Drug-Induced Bile Salt Export Pump (BSEP) Interference [0.03%]
当前药物诱导的胆盐出口泵(BSEP)干扰的概念
J Gerry Kenna
J Gerry Kenna
Numerous drugs have been shown to inhibit the activity of the Bile Salt Export Pump (BSEP in humans, Bsep in animals), and this is now considered to be one of several mechanisms by which idiosyncratic drug-induced liver injury (DILI) may be...
Jennifer R Nash,Greg Mun,Hans A Raabe et al.
Jennifer R Nash et al.
Measuring in vitro cytotoxicity is one method currently used to estimate damage to the eye after chemical exposure. The Cytosensor Microphysiometer method evaluates cytotoxicity by measuring the test material-induced reduction in the metabo...
Comparative human and rat "neurosphere assay" for developmental neurotoxicity testing [0.03%]
人和大鼠的发育神经毒性试验的比较性“神经球测定”法
Jenny Baumann,Marta Barenys,Kathrin Gassmann et al.
Jenny Baumann et al.
The developing nervous system is highly vulnerable to the adverse effects of chemical agents. Currently, there is an increasing need for testing and regulating chemical compounds in general use and, due to the lack of available data, to ide...
Comparative Study
Current protocols in toxicology. 2014 Feb 20:59:12.21.1-24. DOI:10.1002/0471140856.tx1221s59 2014
Identifying Compounds that Induce Opening of the Mitochondrial Permeability Transition Pore in Isolated Rat Liver Mitochondria [0.03%]
用于诱导分离的 rat 肝线粒体 permeability transition孔开放的化合物的鉴定
Lisa Marroquin,Rachel Swiss,Yvonne Will
Lisa Marroquin
The mitochondrial permeability transition pore (MPTP) is a protein pore that forms in the inner mitochondrial membrane and allows the membrane to be permeable to all molecules of less than 1500 Da. Ca(2+), numerous reactive chemicals, and o...
Assessment of fatty acid beta oxidation in cells and isolated mitochondria [0.03%]
细胞和分离线粒体中脂质酸β氧化的评估
George W Rogers,Sashi Nadanaciva,Rachel Swiss et al.
George W Rogers et al.
Fatty acid beta oxidation is a major pathway of energy metabolism and occurs primarily in mitochondria. Drug-induced modulation of this pathway can cause adverse effects such as liver injury, or be beneficial for treating heart failure, typ...
Measuring Mitochondrial Function in Permeabilized Cells Using the Seahorse XF Analyzer or a Clark-Type Oxygen Electrode [0.03%]
采用赛勃氢 XF 分析仪或克劳克型氧电极测定透化细胞的线粒体功能
Ajit S Divakaruni,George W Rogers,Anne N Murphy
Ajit S Divakaruni
Measurements of mitochondrial respiration in intact cells can help define metabolism and its dysregulation in fields such as cancer, metabolic disease, immunology, and neurodegeneration. Although cells can be offered various substrates in t...
Pamela J Roqué,Marina Guizzetti,Lucio G Costa
Pamela J Roqué
Behavioral problems (e.g., learning and memory) following developmental exposure to toxicants suggests that dysregulation of the process of synapse formation and function may occur. The ability to assess these changes is thus of value. This...
High-Content Imaging Assays for Identifying Compounds that Generate Superoxide and Impair Mitochondrial Membrane Potential in Adherent Eukaryotic Cells [0.03%]
一种能够在贴壁真核细胞中生成超氧和破坏线粒体膜电位的化合物的高内涵成像筛选法
Puja Billis,Yvonne Will,Sashi Nadanaciva
Puja Billis
Reactive oxygen species (ROS) are constantly produced in cells as a result of aerobic metabolism. When there is an excessive production of ROS and the cell's antioxidant defenses are overwhelmed, oxidative stress occurs. The superoxide anio...