Seahorse Xfe 24 Extracellular Flux Analyzer-Based Analysis of Cellular Respiration in Caenorhabditis elegans [0.03%]
基于海马Xfe 24外源性通量分析仪的秀丽隐杆线虫细胞呼吸测定
Anthony L Luz,Latasha L Smith,John P Rooney et al.
Anthony L Luz et al.
Mitochondria are critical for their role in ATP production as well as multiple nonenergetic functions, and mitochondrial dysfunction is causal in myriad human diseases. Less well appreciated is the fact that mitochondria integrate environme...
Measuring p66Shc Signaling Pathway Activation and Mitochondrial Translocation in Cultured Cells [0.03%]
测定和测量培养细胞中p66Shc信号通路活化及线粒体易位的方法
Mariusz R Wieckowski,Cláudia M Deus,Renata Couto et al.
Mariusz R Wieckowski et al.
The adaptor protein p66Shc links membrane receptors to intracellular signaling pathways, with downstream consequences on mitochondrial metabolism and reactive oxygen species production. Moreover, p66Shc has also been implicated in cancer de...
Caenorhabditis elegans as a Model for Toxic Effects of Nanoparticles: Lethality, Growth, and Reproduction [0.03%]
秀丽杆线虫纳米粒子毒效应模型：对死亡性、生长性和繁殖性的研究
Laura L Maurer,Ian T Ryde,Xinyu Yang et al.
Laura L Maurer et al.
The nematode Caenorhabditis elegans is extensively utilized in toxicity studies. C. elegans offers a high degree of homology with higher organisms, and its ease of use and relatively inexpensive maintenance have made it an attractive comple...
A Graham Pockley,Gemma A Foulds,Julie A Oughton et al.
A Graham Pockley et al.
Fluorescent immunophenotyping uses fluorescently-conjugated antibodies to identify, characterize and quantify distinct subpopulations of cells within heterogeneous single-cell populations, either in the context of tissue (using fluorescence...
microRNA Profiling as Tool for Developmental Neurotoxicity Testing (DNT) [0.03%]
微小核糖核酸谱分析在发育神经毒性检测中的应用研究
Lena Smirnova,Andrea E M Seiler,Andreas Luch
Lena Smirnova
microRNAs (miRNAs) are small non-coding RNA molecules functioning as post-transcriptional regulators of gene expression. miRNAs play a significant role in organism development, regulating developmental timing, cell differentiation, and spec...
Detection of Bulky Endogenous Oxidative DNA Lesions Derived from 8,5'-Cyclo-2'-deoxyadenosine by ³²P-Postlabeling Assay [0.03%]
用~(32)P后标法测定来源于8,5'-环-2'-脱氧腺苷的端ogenous氧化型DNA bulky损伤部位
Guo-Dong Zhou,Bhagavatula Moorthy
Guo-Dong Zhou
8,5'-Cyclopurine-2'-deoxynucleotides represent a class of oxidative DNA lesions that are specifically repaired by nucleotide excision repair but not by base excision repair or direct enzymatic reversion. The 32P-postlabeling assay is an ult...
High-Resolution Multi-Photon Imaging of Morphological Structures of Caenorhabditis elegans [0.03%]
秀丽线虫精细解剖结构的多光子高分辨率成像研究
Gabriele M Bixel,Stephanie J B Fretham,Michael Aschner
Gabriele M Bixel
In this protocol, we combine two-photon excitation fluorescence with nonlinear optical measurements to reconstruct the three-dimensional architecture of the pharyngeal region and the muscular system of the anterior and mid-body region of Ca...
High-Resolution Multi-Photon Imaging of Morphological Structures of Caenorhabditis elegans [0.03%]
秀丽线虫亚细胞分辨率多光子形态结构影像数据集
Gabriele M Bixel,Stephanie J B Fretham,Michael Aschner
Gabriele M Bixel
In this protocol, we combine two-photon excitation fluorescence with nonlinear optical measurements to reconstruct the three-dimensional architecture of the pharyngeal region and the muscular system of the anterior and mid-body region of Ca...
Samuel W Caito,Michael Aschner
Samuel W Caito
Glutathione (GSH) is the most abundant intracellular thiol with diverse functions from redox signaling, xenobiotic detoxification, and apoptosis. The quantification of GSH is an important measure for redox capacity and oxidative stress. Thi...
Determination of Metabolic Stability Using Cryopreserved Hepatocytes from Rainbow Trout (Oncorhynchus mykiss) [0.03%]
用于代谢稳定性测定的大马哈鱼（Oncorhynchus mykiss）冰冻肝细胞的确定
Kellie A Fay,Diane L Nabb,Robert T Mingoia et al.
Kellie A Fay et al.
Trout provide a relatively easy source of hepatocytes that can be cryopreserved and used for a range of applications including toxicity testing and determination of intrinsic clearance. Standard protocols for isolating, cryopreserving, and ...