Simultaneous Ca2+ Imaging and Optogenetic Stimulation of Cortical Astrocytes in Adult Murine Brain Slices [0.03%]
成年小鼠脑片中皮层星形胶质细胞的钙成像和光遗传学刺激同步研究
Lakshmini Balachandar,Karla A Montejo,Eleane Castano et al.
Lakshmini Balachandar et al.
Astrocytes are actively involved in a neuroprotective role in the brain, which includes scavenging reactive oxygen species to minimize tissue damage. They also modulate neuroinflammation and reactive gliosis prevalent in several brain disor...
Francesca Scarsi,Diego Scheggia,Francesco Papaleo
Francesca Scarsi
Attentional set shifting is a measure of cognitive flexibility and executive functions widely assessed in humans by the Wisconsin Card Sorting Test (WCST) and the CANTAB Intra-/Extra-Dimensional set-shifting task (ID/ED). The recently estab...
A Guide to Fluorescence Lifetime Microscopy and Förster's Resonance Energy Transfer in Neuroscience [0.03%]
荧光寿命显微镜和福斯特共振能量转移在神经科学中的应用指南
Daniel J Liput,Tuan A Nguyen,Shana M Augustin et al.
Daniel J Liput et al.
Fluorescence lifetime microscopy (FLIM) and Förster's resonance energy transfer (FRET) are advanced optical tools that neuroscientists can employ to interrogate the structure and function of complex biological systems in vitro and in vivo ...
Development, Screening, and Validation of Camelid-Derived Nanobodies for Neuroscience Research [0.03%]
骆驼源纳米抗体的开发、筛选和验证用于神经科学研究
Clara E Gavira-O&#x;Neill,Jie-Xian Dong,James S Trimmer
Clara E Gavira-O&#x;Neill
Nanobodies (nAbs) are recombinant antigen-binding variable domain fragments obtained from heavy-chain-only immunoglobulins. Among mammals, these are unique to camelids (camels, llamas, alpacas, etc.). Nanobodies are of great use in biomedic...
James S Trimmer
James S Trimmer
Basic neuroscience research employs antibodies as key reagents to label, capture, and modulate the function of proteins of interest. Antibodies are immunoglobulin proteins. Recombinant antibodies are immunoglobulin proteins whose nucleic ac...
Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model [0.03%]
帕金森病体外模型的自动量化线粒体碎裂的方法
Daniel J Rees,Luke Roberts,M Carla Carisi et al.
Daniel J Rees et al.
Neuronal mitochondrial fragmentation is a phenotype exhibited in models of neurodegeneration such as Parkinson's disease. Delineating the dysfunction in mitochondrial dynamics found in diseased states can aid our understanding of underlying...
Whole-Brain Image Analysis and Anatomical Atlas 3D Generation Using MagellanMapper [0.03%]
使用MagellanMapper进行全脑图像分析和结构三维图谱的生成
David M Young,Clif Duhn,Michael Gilson et al.
David M Young et al.
MagellanMapper is a software suite designed for visual inspection and end-to-end automated processing of large-volume, 3D brain imaging datasets in a memory-efficient manner. The rapidly growing number of large-volume, high-resolution datas...
A Modular Setup to Run a Large Line of Behavioral Testing in Mice in a Single Space [0.03%]
用于在单一空间运行大量行为测试的小鼠模块化设置
Rachel Manno,John Witte,Thomas Papouin
Rachel Manno
Elucidating the complex neural mechanisms that underlie cognition is contingent upon our ability to measure behavioral outputs reliably in animal models. While the development of open-source software has made behavioral science more accessi...
AAV Production Everywhere: A Simple, Fast, and Reliable Protocol for In-house AAV Vector Production Based on Chloroform Extraction [0.03%]
无处不在的AAV生产：基于氯仿提取的一种简单、快速可靠的AAV载体内部生产方案
Matilde Negrini,Gang Wang,Andreas Heuer et al.
Matilde Negrini et al.
Recombinant adeno-associated virus (rAAV) is a mammalian virus that has been altered to be used as a gene delivery vehicle. Several changes to the viral genome have made them replication deficient so that this aspect of the viral infection ...
Caitlin A Orsini,Nicholas W Simon
Caitlin A Orsini
Deficits in decision making are at the heart of many psychiatric diseases, such as substance abuse disorders and attention deficit hyperactivity disorder. Consequently, rodent models of decision making are germane to understanding the neura...