Microfluidics-Assisted TIRF Imaging to Study Single Actin Filament Dynamics [0.03%]
基于微流控的TIRF成像技术研究单个肌动蛋白丝的动力学行为
Shashank Shekhar
Shashank Shekhar
Dynamic assembly of actin filaments is essential for many cellular processes. The rates of assembly and disassembly of actin filaments are intricately controlled by regulatory proteins that interact with the ends and the sides of filaments ...
High Purity Isolation and Sensitive Quantification of Extracellular Vesicles Using Affinity to TIM4 [0.03%]
基于TIM4亲和力的外泌体高效分离与痕量定量研究
Takeshi Yoshida,Takamasa Ishidome,Rikinari Hanayama
Takeshi Yoshida
Almost all types of cells secrete extracellular vesicles (EVs), including exosomes and microvesicles. EVs carry various proteins, lipids, mRNAs, and microRNAs, and may participate in many aspects of physiological and pathophysiological proc...
Live-Cell Visualization of Calcium Flux in Vibratome-Cut Thick Sections of Viable Tumor Tissue [0.03%]
肿瘤组织切片中的钙离子通量的实时观测
James Koh,Joyce A Hogue,Julie A Sosa
James Koh
This unit outlines a live-cell imaging approach developed for visualization of intracellular calcium flux in human parathyroid tumors following stimulation of the calcium-sensing receptor (CASR), a class C G protein-coupled receptor (GPCR)....
Min Zhang,Dawei Liu,Liang Ge
Min Zhang
Autophagy is an essential cellular process for bulk degradation of cytoplasmic components through the lysosome. Underlying this process is an intricate interaction between protein factors and the cell endomembrane system, leading to a gradu...
Souheila Amor,Sunny Y Yang,Judy M Y Wong et al.
Souheila Amor et al.
G-quadruplexes (G4s) are higher-order nucleic acid structures that fold from guanine (G)-rich DNA and RNA strands. This field of research gains traction as a major chemical biology area since it aims at uncovering many key cellular mechanis...
Lectin-Array Blotting [0.03%]
凝集素阵列印迹法
Raquel Pazos,Juan Echevarria,Alvaro Hernandez et al.
Raquel Pazos et al.
Aberrant protein glycosylation is a hallmark of cancer, infectious diseases, and autoimmune or neurodegenerative disorders. Unlocking the potential of glycans as disease markers will require rapid and unbiased glycoproteomics methods for gl...
Centrifugation-Free Magnetic Isolation of Functional Mitochondria Using Paramagnetic Iron Oxide Nanoparticles [0.03%]
利用顺磁性氧化铁纳米颗粒进行无需离心的线粒体功能性磁分离技术
Bhabatosh Banik,Shanta Dhar
Bhabatosh Banik
Subcellular fractionation techniques are essential for cell biology and drug development studies. The emergence of organelle-targeted nanoparticle (NP) platforms necessitates the isolation of target organelles to study drug delivery and act...
Csilla-Maria Ferencz,Gernot Guigas,Andreas Veres et al.
Csilla-Maria Ferencz et al.
Reconstitution of cellular organelles in vitro offers the possibility to perform quantitative and qualitative experiments in a controlled environment that cannot be done with the same accuracy in living cells. Following a previous report, t...
Determination of Membrane Protein Distribution on the Nuclear Envelope by Single-Point Single-Molecule FRAP [0.03%]
通过单点单分子FRAP测定核膜上膜蛋白的分布
Krishna C Mudumbi,Weidong Yang
Krishna C Mudumbi
Nuclear envelope transmembrane proteins (NETs) are synthesized on the endoplasmic reticulum and then transported from the outer nuclear membrane (ONM) to the inner nuclear membrane (INM) in eukaryotic cells. The abnormal distribution of NET...
Brian J DuChez
Brian J DuChez
Cell migration is essential for many biological processes including development, wound healing, and metastasis. However, studying cell migration often requires the time-consuming and labor-intensive task of manually tracking cells. To accel...