Measuring Mitochondrial Respiration in Previously Frozen Biological Samples [0.03%]
测量先前已冷冻生物样本的线粒体呼吸作用
Corey Osto,Ilan Y Benador,Jennifer Ngo et al.
Corey Osto et al.
Measuring oxygen consumption allows for the role of mitochondrial function in biological phenomena and mitochondrial diseases to be determined. Although respirometry has become a common approach in disease research, current methods are limi...
Proximity Ligation Assay for Detecting Protein-Protein Interactions and Protein Modifications in Cells and Tissues in Situ [0.03%]
用于检测细胞和组织中蛋白质-蛋白质相互作用及蛋白质修饰的原位 proximity ligation 实验室检测方法
Marihan Hegazy,Eran Cohen-Barak,Jennifer L Koetsier et al.
Marihan Hegazy et al.
Biochemical methods can reveal stable protein-protein interactions occurring within cells, but the ability to observe transient events and to visualize the subcellular localization of protein-protein interactions in cells and tissues in sit...
Methods for Investigating Corneal Cell Interactions and Extracellular Vesicles In Vitro [0.03%]
研究角膜细胞相互作用和胞外囊泡的体外方法
Tina B McKay,Xiaoqing Guo,Audrey E K Hutcheon et al.
Tina B McKay et al.
Science and medicine have become increasingly "human-centric" over the years. A growing shift away from the use of animals in basic research has led to the development of sophisticated in vitro models of various tissues utilizing human-deri...
Multiplexed Proximity Biotinylation Coupled to Mass Spectrometry for Defining Integrin Adhesion Complexes [0.03%]
用于定义整联蛋白粘附复合物的多路邻近生物素化结合到质谱上
Megan R Chastney,Craig Lawless,Martin J Humphries
Megan R Chastney
BioID, a proximity biotinylation technique, offers a valuable approach to examine the interactions occurring within protein complexes that complements traditional protein biochemical methods. BioID has various advantages that are beneficial...
Preparation of Extracellular Matrix Paper and Construction of Multi-Layered 3D Tissue Model [0.03%]
细胞外基质纸的制备及多层三维组织模型的构建
Hirotaka Nakatsuji,Shiro Kitano,Shinji Irie et al.
Hirotaka Nakatsuji et al.
Construction of organized three-dimensional (3D) tissue with extracellular matrix (ECM) and multiple types of cells is important for tissue engineering to enable tissue function and enhance cellular function. However, the concentration of E...
Metabolic Analysis at the Nanoscale with Multi-Isotope Imaging Mass Spectrometry (MIMS) [0.03%]
利用多同位素成像质谱技术(MIMS)进行纳米级代谢分析
Derek P Narendra,Matthew L Steinhauser
Derek P Narendra
Incorporation of a stable-isotope metabolic tracer into cells or tissue can be followed at submicron resolution by multi-isotope imaging mass spectrometry (MIMS), a form of imaging secondary ion microscopy optimized for accurate isotope rat...
Exosome Isolation by Ultracentrifugation and Precipitation and Techniques for Downstream Analyses [0.03%]
超速离心和沉淀法分离外泌体及下游分析技术
Christina Coughlan,Kimberley D Bruce,Olivier Burgy et al.
Christina Coughlan et al.
Exosomes are 50- to 150-nm-diameter extracellular vesicles secreted by all mammalian cells except mature red blood cells and contribute to diverse physiological and pathological functions within the body. Many methods have been used to isol...
Jean-Loup Duband,Nioosha Nekooie-Marnany,Sylvie Dufour
Jean-Loup Duband
Neural crest cells constitute a unique population of progenitor cells with extensive stem cell capacities able to navigate throughout various environments in the embryo and are a source of multiple cell types, including neurons, glia, melan...
QuoVadoPro, an Autonomous Tool for Measuring Intracellular Dynamics using Temporal Variance [0.03%]
基于时间方差的测量细胞内动力学的自主工具QuoVadoPro
Himanish Basu,Thomas L Schwarz
Himanish Basu
Trafficking of intracellular cargo is essential to cellular function and can be defective in pathological states including cancer and neurodegeneration. Tools to quantify intracellular traffic are thus necessary for understanding this funda...
Kymolyzer, a Semi-Autonomous Kymography Tool to Analyze Intracellular Motility [0.03%]
一种半自主细胞内运动分析工具Kymolyzer及其克氏成像技术
Himanish Basu,Lai Ding,Gulcin Pekkurnaz et al.
Himanish Basu et al.
The movement of intracellular cargo, such as transcripts, proteins, and organelles, is fundamental to cellular function. Neurons, due to their long axons and dendrites, are particularly dependent on proper intracellular trafficking and vuln...