The Sandwich Assay: A Method for Subcellular Visualization of Paracellular Macromolecule Passage in Epithelial Sheets [0.03%]
夹心测定法：一种用于上皮细胞层跨细胞大分子转运的亚细胞可视化方法
Juliane Reiche,Michael Schumann,Jan F Richter
Juliane Reiche
To date, the permeability of epithelia to larger solutes (greater than ∼4 Å in diameter) has been analyzed by flux measurements using various tracers that cannot spatially resolve the permeation sites. This unit describes a method for loc...
Zhilin Yang,Heejun Choi
Zhilin Yang
Detection of reactive oxygen species (ROS) in bacteria has been limited to bulk biochemical assays. Although they are powerful and quantitative tools to understand the overall production of ROS in E. coli, such assays provide limited spatia...
Anne Woods,John R Couchman
Anne Woods
Proteoglycans can be difficult molecules to isolate and analyze due to large mass, charge, and tendency to aggregate or form macromolecular complexes. This unit describes detailed methods for purification of matrix, cell surface, and cytosk...
Keishi Shintomi,Tatsuya Hirano
Keishi Shintomi
The mitotic chromosome, which is composed of a pair of sister chromatids, is a large macromolecular assembly that ensures faithful transmission of genetic information into daughter cells. Despite its fundamental importance, how a nucleosome...
Transcription Factor-Mediated Differentiation of Human iPSCs into Neurons [0.03%]
转录因子介导的人诱导多能干细胞向神经元分化
Michael S Fernandopulle,Ryan Prestil,Christopher Grunseich et al.
Michael S Fernandopulle et al.
Accurate modeling of human neuronal cell biology has been a long-standing challenge. However, methods to differentiate human induced pluripotent stem cells (iPSCs) to neurons have recently provided experimentally tractable cell models. Nume...
Using Acutely Dissociated and Purified Oligodendrocyte Precursor Cells for High-Throughput Drug Screening to Identify Compounds that Promote Oligodendrocyte Differentiation [0.03%]
用于高通量药物筛选的急性分离和纯化的少突胶质细胞前体细胞促进少突胶质细胞分化的化合物的鉴定
Karen Lariosa-Willingham,Dmitri Leonoudakis
Karen Lariosa-Willingham
Multiple sclerosis (MS) is an autoimmune disease that involves an immune-mediated inflammatory response in the central nervous system and optic nerve resulting in demyelination and neural degeneration, the cause of which is unknown. The adu...
Monitoring the Effects of Pharmacological Reagents on Mitochondrial Morphology [0.03%]
药理试剂对线粒体形态影响的监测方法研究
Dong Fu,Jennifer Lippincott-Schwartz
Dong Fu
This protocol describes how to apply appropriate pharmacological controls to induce mitochondrial fusion or fission in studies of mitochondria morphology for four different mammalian cell types, HepG2 human liver hepatocellular carcinoma ce...
Isolation and Enrichment of Bacteriophages by Membrane Filtration Immobilization Technique [0.03%]
膜过滤固定化技术的噬菌体的分离与纯化
Gaurav S Ghugare,Vijay D Nimkande,Krishna Khairnar
Gaurav S Ghugare
The method described here enables rapid bacteriophage isolation and enrichment of host-specific bacteriophages from an environmental sample. This is achieved by using a simple 0.45-µm Millipore membrane where a specific host is immobilized...
Sarah Cohen,Alex M Valm,Jennifer Lippincott-Schwartz
Sarah Cohen
Fluorescent proteins and vital dyes are invaluable tools for studying dynamic processes within living cells. However, the ability to distinguish more than a few different fluorescent reporters in a single sample is limited by the spectral o...
Self-Pressurized Rapid Freezing as Cryo-Fixation Method for Electron Microscopy and Cryopreservation of Living Cells [0.03%]
自加压快速冷冻法在电子显微镜和活细胞 cryopreservation 中的 cryo-固定方法
Jan Huebinger,Markus Grabenbauer
Jan Huebinger
Reduction or complete prevention of ice crystal formation during freezing of biological specimens is mandatory for two important biological applications: (1) cryopreservation of living cells or tissues for long-term storage, and (2) cryo-fi...