Quantifying the Proteolytic Cleavage of Plasma Membrane Proteins in Living Cells [0.03%]
活细胞中膜蛋白内吞作用的量化研究
Martino Calamai,Francesco Saverio Pavone
Martino Calamai
The standard approach to study the activity of proteases consists of lysing cells and measuring the changes in the fluorescence properties of a synthetic substrate after cleavage in vitro. Here, a general protocol that uses a bi-fluorescent...
Imaging Membrane Repair in Single Cells Using Correlative Light and Electron Microscopy [0.03%]
应用相关光镜和电子显微镜技术研究单细胞中的膜修复过程
Coralie Croissant,Flora Bouvet,Sisareuth Tan et al.
Coralie Croissant et al.
Many cells possess the ability to repair plasma membrane disruption in physiological conditions. Growing evidence indicates a correlation between membrane repair and many human diseases. For example, a negative correlation is observed in mu...
Expansion Microscopy: Protocols for Imaging Proteins and RNA in Cells and Tissues [0.03%]
用于细胞和组织中蛋白质及RNA成像的膨胀显微镜实验方案
Shoh M Asano,Ruixuan Gao,Asmamaw T Wassie et al.
Shoh M Asano et al.
Expansion microscopy (ExM) is a recently developed technique that enables nanoscale-resolution imaging of preserved cells and tissues on conventional diffraction-limited microscopes via isotropic physical expansion of the specimens before i...
Hepatitis C Virus Infection of Cultured Cells and Primary Human Hepatocytes [0.03%]
体外培养细胞和原代人肝细胞的丙型肝炎病毒感染
Brianna Lowey,Qisheng Li
Brianna Lowey
Hepatitis C virus (HCV) is a positive-sense, single-stranded RNA virus in the family Flaviviridae with specific hepatotropism. HCV poses a significant health burden worldwide, frequently causing chronic infections associated with progressiv...
Soraya Mezouar,Amira Ben Amara,Joana Vitte et al.
Soraya Mezouar et al.
Mast cells have been identified as resident cells of human placental tissue by immunohistological procedures, suggesting that they may play a role in pregnancy. However, the study of placental mast cells requires their isolation. Here, we d...
Natalya Khozhukhar,Domenico Spadafora,Yelitza Rodriguez et al.
Natalya Khozhukhar et al.
To cope with DNA damage, mitochondria developed a pathway by which severely damaged or unrepairable mitochondrial DNA (mtDNA) molecules are abandoned and degraded, and new molecules are resynthesized using intact templates, if available. In...
Yun Xiong,Ying Zhang,Jun Yao et al.
Yun Xiong et al.
Mass spectrometry-based proteomic technology experienced remarkable advancement in the past decades. However, their application was still hampered by the complexity of sample preparation. Conventional strategies for sample preparation incor...
Live-Cell Imaging and Analysis with Multiple Genetically Encoded Reporters [0.03%]
基于多种基因编码报告基因的活细胞成像与分析
Michael Pargett,John G Albeck
Michael Pargett
Genetically encoded live-cell reporters measure signaling pathway activity at the cellular level with high temporal resolution, often revealing a high degree of cell-to-cell heterogeneity. By using multiple spectrally distinct reporters wit...
Visualizing Autophagic Lysosome Reformation in Cells Using In Vitro Reconstitution Systems [0.03%]
利用体外重组系统在细胞中可视化自噬溶酶体重塑现象
Yang Chen,Qian Peter Su,Yujie Sun et al.
Yang Chen et al.
Autophagy is a lysosome-based degradation pathway. Autophagic lysosome reformation (ALR) is a lysosomal membrane recycling process that marks the terminal step of autophagy. During ALR, LAMP1-positive tubules, named reformation tubules, are...
Mobile Quantitative Colocalization Analysis of Fluorescence Microscopy Images [0.03%]
荧光显微图像的移动定量共定位分析方法研究
Vadim Zinchuk,Olga Grossenbacher-Zinchuk
Vadim Zinchuk
Understanding the nature of fluorescently-labelled molecules requires proper quantification of their colocalization. We developed a new approach to enable quantification of colocalization of markers in fluorescence microscopy images using m...