Enzymatic amplification of DNA by PCR: standard procedures and optimization [0.03%]
DNA的酶促扩增(PCR):标准程序及优化方法
M F Kramer,D M Coen
M F Kramer
This appendix describes a method for amplifying DNA enzymatically by the polymerase chain reaction (PCR), including procedures to quickly determine conditions for successful amplification of the sequence and primer sets of interest, and to ...
Silanizing glassware [0.03%]
玻璃器皿的硅烷化处理
B Seed
B Seed
This unit provides a protocol for silanizing (siliconizing) glassware to prevent adsorption of solute to the glass surface and to increase the hydrophobicity of the surface. This is particularly important when dealing with low concentration...
Quantification of DNA and RNA with absorption and fluorescence spectroscopy [0.03%]
吸收及荧光光谱法测定DNA和RNA浓度
S R Gallagher
S R Gallagher
Reliable quantitation of nanogram and microgram amounts of DNA and RNA in solution is essential to researchers in cell and molecular biology. In addition to the traditional absorbance measurements at 260 nm, two more sensitive fluorescence ...
S M Andrew,J A Titus,L Zumstein
S M Andrew
Conventional dialysis separates small molecules from large molecules by allowing diffusion of only the small molecules through selectively permeable membranes. Dialysis is usually used to change the salt (small-molecule) composition of a ma...
Molecular biology techniques [0.03%]
分子生物学技术
Protocols described in Current Protocols in Cell Biology may include molecular biology techniques and biochemical techniques that are not fully described in this book. Although it may be reasonable to assume that cell biologists have at lea...
Standard laboratory equipment [0.03%]
标准实验室设备
This appendix lists pieces of equipment that are standard in the modern cell biology laboratory-i.e., items used extensively in this manual and thus not usually included in the individual materials lists.
Medium formulations [0.03%]
中等表述方式
This appendix lists the formulations for four common tissue culture media: DMEM, Ham's F-12 nutrient mixture, MEM and RPMI 1640.
This section describes the preparation of buffers and reagents used in this manual for cell culture, manipulation of tissue, and cell biological methods. Also discussed are special considerations for PCR experiments and for working with RNA...
A D Baxevanis,B F Ouellette
A D Baxevanis
With the explosion of sequence and structural information available to researchers, the field of bioinformatics is playing an increasingly large role in the study of fundamental biomedical problems. This information is generally available t...
Absorption and emission maxima for common fluorophores [0.03%]
常见荧光团的吸收和发射峰$maxima$可以译为“最值”、“峰值”等，在此上下文中可省略不译。
B Herman
B Herman
This unit lists absorption and emission maximum wavelengths for common fluorophores used in cell biology. This information can be used in designing experiments and selecting appropriate filters for use with the fluorophores.