Initiation, Expansion, and Cryopreservation of Human Primary Tissue-Derived Normal and Diseased Organoids in Embedded Three-Dimensional Culture [0.03%]
基于嵌入式三维培养的人源原代正常和病变器官样组织的诱导、扩增及冻存技术
James Clinton,Penney McWilliams-Koeppen
James Clinton
Organoids are primary patient-derived micro tissues grown within a three-dimensional extracellular matrix that better represents in vivo physiology and genetic diversity than existing two-dimensional cell lines. Organoids rely on the self-r...
Isolation, Culture, and Differentiation of Mammary Epithelial Stem/Progenitor Cells from Fresh or Ex Vivo Cultured Human Breast Tissue [0.03%]
新鲜或体外培养的人类乳腺组织中分离、培养和诱导分化乳腺上皮干细胞/前体细胞
Guang Chen,Hakim Bouamar,Lu-Zhe Sun
Guang Chen
In vivo transplantation is the gold standard method for characterization of stem/progenitor cell self-renewal, tissue regeneration, and tumorigenesis. The method requires an enriched population of stem cells that represent a small fraction ...
Identification, Localization, and Quantification of HIV Reservoirs Using Microscopy [0.03%]
利用显微镜技术识别、定位和量化艾滋病毒储存库
Lisa Prevedel,Nancy Ruel,Paul Castellano et al.
Lisa Prevedel et al.
The major barrier to eradicating human immunodeficiency virus-1 (HIV) infection is the generation and extended survival of HIV reservoirs. In order to eradicate HIV infection, it is essential to detect, quantify, and characterize circulatin...
In vitro Assays for Targeting and Insertion of Tail-Anchored Proteins Into the ER Membrane [0.03%]
针对尾部锚定蛋白进入内质网膜的定位和插入的体外测定法
Hyunju Cho,Un Seng Chio,Shu-Ou Shan
Hyunju Cho
Membrane proteins mediate numerous essential cellular functions. Due to the aggregation propensity of hydrophobic transmembrane domains in aqueous environments, the targeting and insertion of membrane proteins pose major challenges to cells...
Monitoring Sphingolipid Trafficking in Cells using Fluorescence Microscopy [0.03%]
利用荧光显微镜技术监测细胞中的鞘脂运输
Emma L Sundberg,Yongqiang Deng,Christopher G Burd
Emma L Sundberg
Sphingolipids are structural components of organelle membranes that also participate in signal transduction pathways. Complex sphingolipids are trafficked from their site of synthesis in organelles of the early secretory pathway to the Golg...
An On-Chip Method for Long-Term Growth and Real-Time Imaging of Brain Organoids [0.03%]
芯片上大脑类器官的长期培养及实时成像方法
Eyal Karzbrun,Rami Yair Tshuva,Orly Reiner
Eyal Karzbrun
Brain organoids are an emerging technique for studying human neurodevelopment in vitro, with biomedical implications. However, three-dimensional tissue culture poses several challenges, including lack of nutrient exchange at the organoid co...
Bradford Hall,Andrew Cho,Advait Limaye et al.
Bradford Hall et al.
CRISPR/Cas9 technology has revolutionized genome editing in mice, allowing for simple and rapid development of knockouts and knockins. CRISPR relies on small guide RNAs that direct the RNA-guided nuclease Cas9 to a designated genomic site u...
Hao Feng,Baochi Ou,Wei Dong et al.
Hao Feng et al.
Co-cultivation of tumor cells and liver resident immune cells or other non-parenchymal cells (NPCs) from the same donor is important for the study of cancer metastasis. So far, little is known about the mechanism of tumor cell or pathogen c...
Kentaro Hozumi,Motoyoshi Nomizu
Kentaro Hozumi
Many cell-adhesive peptides have been identified from extracellular matrix (ECM) proteins, such as collagen, fibronectin, laminin, and vitronectin. ECM proteins have various cell-adhesive sequences. Most peptides demonstrate cell-adhesive a...
Triggered Cell-Cell Fusion Assay for Cytoplasmic and Organelle Intermixing Studies [0.03%]
用于细胞质和细胞器相互混合研究的诱导型细胞融合分析方法
Daniel Feliciano,Jonathon Nixon-Abell,Jennifer Lippincott-Schwartz
Daniel Feliciano
Different multicellular organisms undergo cell-cell fusion to form functional syncytia that support specialized functions necessary for proper development and survival. For years, monitoring the structural consequences of this process using...