Preparation of extracellular matrices produced by cultured corneal endothelial and PF-HR9 endodermal cells [0.03%]
培养的角膜内皮和PF-HR9肠上皮细胞外基质的制备
I Vlodavsky
I Vlodavsky
The ECM is an organized complex of collagens, proteoglycans, and glycoproteins, all interacting to produce a highly stable structure upon which cells migrate, proliferate, differentiate, and survive in vivo. Cultured bovine corneal endothel...
H K Kleinman
H K Kleinman
Gelled substrates can be used for a variety of in vitro and in vivo experiments. A type I collagen gelled substrate will promote cell growth and differentiation. Gelled Matrigel substrate promotes the survival of explanted cells and tissues...
H K Kleinman
H K Kleinman
This unit describes methods for passaging and harvesting the basement membrane matrix-producing EHS tumor and for the subsequent isolation of a crude mixture of basement membrane components termed Matrigel, which promotes the differentiatio...
R P Mecham
R P Mecham
The extracellular matrix provides an environment for cells. It is produced, assembled and modified by cells and in turn, it modifies the functions and behavior of the cells it encounters. The molecules that make up the matrix are diverse in...
Media and culture of yeast [0.03%]
酵母的媒体与文化
S R Green,C M Moehle
S R Green
The yeast, Saccharyomyces cerevisiae, has become an important organism in molecular, biochemical, and genetic analysis. The organism has specific requirements for growth under a variety of conditions. The media, both liquid and solid, simpl...
R Coté
R Coté
This unit describes how to detect and recognize bacterial, fungal, or mycoplasma contamination and how to verify the presence of mycoplasma. Strategies are described for dealing with culture contamination, if necessary.
Sterilization and filtration [0.03%]
灭菌与过滤
R J Coté
R J Coté
This unit on sterility in the tissue culture environment describes methods for sterilization of liquid and dry goods used for tissue culture and filtration of liquids to prevent contamination of cultures.
R J Coté
R J Coté
This unit describes some of the ways that a laboratory can deal with the constant threat of microbial contamination in cell cultures. A protocol on aseptic technique is described first. This catch-all term universally appears in any set of ...
J D Sato,M Kan
J D Sato
When mammalian cells are cultured in vitro, the investigator is attempting to reproduce the physiological environment in order to maintain and analyze normal functions and responses. The culture medium is an essential component of the in vi...
Micro RT-PCR [0.03%]
微RT-PCR技术
C P Paweletz,L Charboneau,L A Liotta
C P Paweletz
This unit presents a collection of protocols for the microisolation, manipulation, and amplification of the RNA content of microdissected cells. Even though emphasis in these protocols is given for microdissected cells, these protocols have...