C Enns
C Enns
Proteins migrate from the site of their synthesis to intracellular, surface, or extracellular sites depending on their function. This well-illustrated overview provides an introduction to the pathways of protein trafficking. It includes des...
Phosphoamino acid analysis [0.03%]
磷酸氨基酸分析
B M Sefton
B M Sefton
Proteins involved in signal transduction are often phosphorylated. Determination of the specific amino acid residue(s) involved is used in characterizing the particular pathway. Partial acid hydrolysis of phosphorylated proteins followed by...
Labeling cultured cells with 32Pi and preparing cell lysates for immunoprecipitation [0.03%]
用32Pi标记培养细胞并制备免疫沉淀的细胞提取液
B M Sefton
B M Sefton
Signal transduction pathways may involve protein phosphorylation at one or more residues. Detection of phosphorylation involves labeling with radioactive inorganic phosphate and subsequent immunoprecipitation with appropriate antibodies. Th...
Z Yao,R Seger
Z Yao
Incorporation of phosphates into serine, threonine, and tyrosine acceptors in proteins is a common mechanism for regulating protein function. This unit presents protocols that use specific anti-phosphoamino acid (PAA) and anti-phosphoprotei...
B M Sefton
B M Sefton
Phosphorylation is the most common and important mechanism of acute and reversible regulation of protein function. Studies of mammalian cells metabolically labeled with [(32)P]orthophosphate suggest that as many as one-third of all cellular...
Movement of nuclei [0.03%]
核的移动
S Reinsch
S Reinsch
This unit describes the first assay that reconstructs the movement of the female pronucleus in the newly fertilized frog egg. Nuclei are assembled in frog egg extracts and translocated along microtubules using the microtubule motor dynein.
Organelle motility in plant cells: imaging golgi and ER dynamics with GFP [0.03%]
植物细胞内质运动：用GFP观察高尔基体和内质网动态变化
C Hawes,F Brandizzil,H Batoko et al.
C Hawes et al.
By the addition of plant or mammalian targeting sequences, green fluorescent protein (GFP) can be directed to the endoplasmic reticulum, the Golgi apparatus, or both organelles in plant cells. This unit describes the application of rapid Ag...
In vitro motility assay to study translocation of actin by myosin [0.03%]
体外肌动蛋白滑行实验研究肌球蛋白对肌动蛋白的移动作用
J R Sellers
J R Sellers
A basic property of myosin is its ability to interact with and translocate actin. This unit describes an in vitro motility assay that can be used to study the translocation, or sliding, of actin filaments by myosin bound to a coverslip. The...
C M Waterman-Storer
C M Waterman-Storer
This unit describes an in vitro assay that uses video-enhanced differential interference contrast (VE-DIC) microscopy to examine the motile interactions between isolated organelle fractions and microtubules (MTs). The method can be used to ...
C A Parent
C A Parent
This unit describes culturing and imaging of D. discoideum amoebae to study fundamental cellular responses, such as motility and directed migration. The system displays powerful molecular genetics that can be used to link structural determi...