D G Edmondson,S Y Roth
D G Edmondson
Far Western blotting is a method for detecting protein-protein interactions. This technique utilizes a nonantibody protein probe to detect interacting proteins immobilized on a membrane support. Proteins to be assayed can be prepared by mul...
Imaging protein-protein interactions by fluorescence resonance energy transfer (FRET) microscopy [0.03%]
利用荧光共振能量转移(FRET)显微镜技术成像蛋白质-蛋白质相互作用
F S Wouters,P I Bastiaens
F S Wouters
FRET microscopy enables the detection of different biochemical states of proteins in cells. The use of fluorescence in the detection of proteins, by chemical modification, by immunofluorescence, or by genetic encoding of a green fluorescent...
R P Haugland
R P Haugland
Antibody conjugates are extremely useful reagents for probing many biologically and chemically important molecules in vitro or in vivo. This unit presents some basic protocols for conjugating antibodies with fluorescent dyes, with biotin, a...
S M Andrew,J A Titus
S M Andrew
While unpurified antibodies are suitable for a number of applications, purified antibodies are required for assays based on known concentration of antibody, for chemical modifications such as radiolabeling or conjugation with fluorochromes,...
H M Cooper,Y Paterson
H M Cooper
Polyclonal antibody preparations contain multiple antibody molecules recognizing different epitopes on an antigen molecule. Good quality polyclonal antisera are useful for a number of applications-e.g., immunoprecipitation, immunoblotting, ...
W M Yokoyama
W M Yokoyama
Monoclonal antibodies are particularly specific for a single epitope and can be produced in large quantities. When immune splenic cells are fused with a tumor cell line, they produce hybridomas each secreting one highly specific antibody. T...
Joshua H Lipschutz,Lucy Erin O&#x;Brien,Yoram Altschuler et al.
Joshua H Lipschutz et al.
Spatial asymmetry is fundamental to the structure and function of most eukaryotic cells. A basic aspect of this polarity is that the cell's plasma membrane is divided into discrete domains. The best studied and simplest example of this occu...
J Neefjes
J Neefjes
Many integral membrane proteins synthesized in the endoplasmic reticulum ultimately arrive at the cell surface to contact the cell environment. During transit through the Golgi and trans-Golgi network, proteins acquire post-translational mo...
T E McGraw,A Subtil
T E McGraw
Many integral membrane proteins synthesized in the endoplasmic reticulum ultimately arrive at the cell surface to contact the cell environment. During transit through the Golgi and trans-Golgi network, proteins acquire post-translational mo...
H H Freeze
H H Freeze
As proteins transit through the cell secretory pathway, modification of their substituent sugar chains occurs in a stepwise fashion. In the course of this processing (maturation) of oligosaccharide chains, the chains acquire sensitivity or ...