H K Kleinman,M C Cid
H K Kleinman
Endothelial cells, which line blood vessels, can be prepared from a variety of tissues. They are frequently prepared from the umbilical vein, which is relatively easy to obtain. The procedure is clearly described and provides a large popula...
W E Biddison
W E Biddison
This unit presents protocols for preparation of lymphocytes from peripheral (whole) blood or leucopherisis samples, first by differential centrifugation in Ficoll-Hypaque to separate them from erythrocytes and granulocytes. Repeated centrif...
A Takashima
A Takashima
Fibroblasts are fairly easily isolated from a variety of tissues, in this case skin. The cells are fast growing and can be rapidly expanded from small samples. They are suitable for behavioral, functional, biochemical, and genomic studies. ...
Michael Elkin,Israel Vlodavsky
Michael Elkin
The most damaging change during cancer progression is the switch from a locally growing tumor to a metastatic killer. This switch involves numerous alterations that allow tumor cells to complete the complex series of events needed for metas...
Cloud P Paweletz,Lu Charboneau,Lance A Liotta
Cloud P Paweletz
During tumor progression, cells acquire genetic and proteomic changes as they transform from normal to hyperplastic, through dysplasia, to carcinoma in situ, and finally to invasive and metastatic. The time course of progression may extend ...
Steven S S Poon,Peter M Lansdorp
Steven S S Poon
This unit describes a quantitative technique for measuring the lengths of telomere repeat sequences in individual chromosomes from single metaphase cells. The technique is based on fluorescence in situ hybridization (FISH) adapted for use w...
Assessment of apoptosis and necrosis by DNA fragmentation and morphological criteria [0.03%]
应用DNA断裂和形态学标准检测细胞凋亡与坏死
Boris Zhivotosky,Sten Orrenius
Boris Zhivotosky
Apoptotic cells share a number of common features, such as phosphatidylserine (PS) exposure, cell shrinkage, chromatin cleavage, nuclear condensation, and formation of pyknotic bodies of condensed chromatin. Necrotic cells exhibit nuclear s...
S H Kaufmann,T J Kottke,L M Martins et al.
S H Kaufmann et al.
This unit describes three methods for the detection of caspase activation as cells undergo apoptosis. Simple and relatively quantitative enzymatic assays are provided using suitable substrates. Because the various low-molecular-weight subst...
B Zhivotovsky,S Orrenius
B Zhivotovsky
This unit attempts to define cell toxicity that leads to cellular demise, with a strong emphasis on cell death via both apoptosis and necrosis, by summarizing some of the more recent developments in cellular, molecular, and biochemical stud...
E A Golemis,I Serebriiskii,R L Finley Jr et al.
E A Golemis et al.
This unit presents protocols designed to detect interacting proteins. Using yeast as a "test tube" and transcriptional activation of a reporter system, interacting proteins can be identified. The system can also be used to test complex form...