Isolation of lysosomes from tissues and cells by differential and density gradient centrifugation [0.03%]
差速及密度梯度离心法分离组织和细胞的溶酶体
J M Graham
J M Graham
This unit covers the use of Percoll, Nycodenz, and iodixanol for purification of lysosomes from a light mitochondrial pellet or postnuclear supernatant. The first protocol describes isolation of lysosomes from rat liver using a Percoll grad...
Isolation of peroxisomes from tissues and cells by differential and density gradient centrifugation [0.03%]
差速及密度梯度离心法分离组织和细胞内的过氧化物体
J M Graham
J M Graham
Peroxisome purification depends on a two-step procedure: differential centrifugation to prepare a light mitochondrial fraction and fractionation on a density-gradient medium preferably iodixanol or Nycodenz, to isolate the peroxisome enrich...
Purification of a crude mitochondrial fraction by density-gradient centrifugation [0.03%]
用密度梯度离心法纯化粗线粒体组分
J M Graham
J M Graham
Most mitochondria prepared by differential centrifugation are contaminated to some extent by lysosomes, peroxisomes, tubular Golgi membranes, and small amounts of endoplasmic reticulum. Density gradient centrifugation using a variety of den...
Isolation of mitochondria from tissues and cells by differential centrifugation [0.03%]
差速离心法分离组织和细胞中的线粒体
J M Graham
J M Graham
Differential centrifugation is used to prepare a "heavy" mitochondrial fraction from liver. These mitochondria are relatively pure, highly coupled, and suitable for respiratory studies. This unit presents protocols for isolation of beef hea...
Isolation of rat hepatocyte plasma membrane sheets and plasma membrane domains [0.03%]
大鼠肝细胞等容素膜片和等容膜结构的分离
P L Tuma,A L Hubbard
P L Tuma
This unit describes a method for isolation of plasma membrane sheets from rat liver. It also includes protocols for preparation of plasma membrane domains isolated from plasma membrane sheets and indirect immunofluorescence localization of ...
John M Graham
John M Graham
The vast majority of methods for the isolation of nuclei and nuclear membranes use soft mammalian tissues (particularly rat liver) as the biological source; however, the protocols described in this unit should be applicable generally to any...
Overview of cell fractionation [0.03%]
细胞分离概论
J D Castle
J D Castle
Cell fractionation is a useful preparative and analytical method in cell biology. It is essential for analysis of composition and function of cellular compartments and it is used to prepare materials for in vitro reconstitution studies This...
P L McNeil
P L McNeil
Techniques for introducing normally impermeant macromolecules into the living cell-referred to as "cell-loading techniques"-are useful in a variety of settings for the cell biologist. Microinjection is probably the most commonly used techni...
L Charboneau,C P Paweletz,L A Liotta
L Charboneau
This unit describes laser capture microdissection (LCM) using the Pixcell II as a technique to provide the scientific community with the opportunity to perform molecular analyses on pure cell populations procured directly from tissues. Afte...
Generation of continuously growing B cell lines by epstein-barr virus transformation [0.03%]
Epstein-B病毒转化生成持续增殖的B细胞系
W E Biddison
W E Biddison
Primary cultures of cells tend to have a limited life span, which in turn limits the availability of a consistent population of cells to study. In this unit, Epstein-Barr virus produced by a marmoset cell line is used to transform B cells i...