Generation of Pancreatic Ductal Organoids and Whole-Mount Immunostaining of Intact Organoids [0.03%]
胰腺导管器官样组织的生成及完整器官样组织的整片染色技术
Habib Rezanejad,Jennifer Hollister Lock,Brooke A Sullivan et al.
Habib Rezanejad et al.
Traditionally, studies of cells and tissues have been performed on isolated primary cells or immortalized cell lines by culturing them in 2D culture dishes or flasks. However, a caveat regarding 2D culture is that the cells poorly recapitul...
Daniel N Itzhak,Julia P Schessner,Georg H H Borner
Daniel N Itzhak
Eukaryotic cells are highly compartmentalized and protein subcellular localization critically influences protein function. Identification of the subcellular localizations of proteins and their translocation events upon perturbation has most...
Anahi Capmany,Bruno Latgé,Kristine Schauer
Anahi Capmany
The consequences of alterations in the distribution of intracellular organelles, observed in many diseases, are often not clear. Intracellular organelles alter their morphology and positioning to regulate cell homeostasis and function. We o...
Jason C Casler,Benjamin S Glick
Jason C Casler
Budding yeast is an excellent model organism for studying the dynamics of the Golgi apparatus. To characterize Golgi function, it is important to visualize secretory cargo as it traverses the secretory pathway. We describe a recently develo...
Single Molecule RNA FISH (smFISH) in Whole-Mount Mouse Embryonic Organs [0.03%]
单分子RNA原位杂交（smFISH）在整胚器官中的应用
Shaohe Wang
Shaohe Wang
Single molecule RNA fluorescence in situ hybridization (smFISH) has become the standard tool for high spatial resolution analysis of gene expression in the context of tissue organization. This article describes protocols to perform smFISH o...
Zeinab F Hosseini,Deirdre A Nelson,Nicholas Moskwa et al.
Zeinab F Hosseini et al.
Organoids are important research tools for studying organ morphogenesis and differentiation because they recapitulate ex vivo the native 3D organization of cells that is essential for proper cell and organ function. The composition of organ...
Huan Hu,Xiaojing Yang,Chao Tang
Huan Hu
Tracking the dynamics of genomic loci is essential for understanding a variety of cellular processes. However, earlier methods have all suffered from a low signal-to-background ratio (SBR), mainly caused by the background fluorescence from ...
Studying Glycolytic Oscillations in Individual Yeast Cells by Combining Fluorescence Microscopy with Microfluidics and Optical Tweezers [0.03%]
结合荧光显微镜、微流控技术及光学钳在单酵母细胞水平上研究糖酵解振荡现象
Anna-Karin Gustavsson,Amin A Banaeiyan,David D van Niekerk et al.
Anna-Karin Gustavsson et al.
In this unit, we provide a clear exposition of the methodology employed to study dynamic responses in individual cells, using microfluidics for controlling and adjusting the cell environment, optical tweezers for precise cell positioning, a...
A Paul Mould
A Paul Mould
In this unit, methods for the analysis of integrin-dependent adhesion are described. Two major types of assays are commonly used for this analysis. The first are cell adhesion assays. A key application of this type of assay is to identify w...
Fluorescence Activated Cell Sorting (FACS) in Genome-Wide Genetic Screening of Membrane Trafficking [0.03%]
流式细胞分选术在膜转运基因组学筛选中的应用研究
Bridget L Menasche,Lauren Crisman,Daniel R Gulbranson et al.
Bridget L Menasche et al.
About one-third of cellular proteins in eukaryotic cells are localized to membrane-enclosed organelles in the endomembrane system. Trafficking of these membrane proteins (including soluble lumenal proteins) among the organelles is mediated ...