Sylvia M Wilson,Ann F Chambers
Sylvia M Wilson
Experimental metastasis assays are used to measure the ability of cancer cells to grow in secondary organs following injection of the cells into the circulation of an experimental animal. The chicken embryo provides an alternative to the mo...
Piotr Pozarowski,Jerzy Grabarek,Zbigniew Darzynkiewicz
Piotr Pozarowski
Common methods applicable to flow cytometry make it possible to: (1) identify and quantify dead or dying cells, (2) reveal a mode of cell death (apoptosis or necrosis), and (3) study mechanisms involved in cell death. Gross changes in cell ...
Nonisotopic methods for determination of poly(ADP-ribose) levels and detection of poly(ADP-ribose) polymerase [0.03%]
测定聚(ADP-核糖)水平和检测多(ADP-核糖)聚合酶的非同位素方法
Jean François Haince,Guy G Poirier,Jim B Kirkland
Jean François Haince
Poly(ADP-ribosyl)ation is a post-translational modification catalyzed mostly by the 116-kDa enzyme poly(ADP-ribose) polymerase-1 (PARP-1), a nuclear enzyme that transfers an ADP-ribose moiety onto a limited number of nuclear proteins, inclu...
Adrián Velázquez-Campoy,Hiroyasu Ohtaka,Azin Nezami et al.
Adrián Velázquez-Campoy et al.
In the last two decades, isothermal titration calorimetry (ITC) has become the preferred technique to determine the binding energetics of biological processes, including protein-ligand binding, protein-protein binding, DNA-protein binding, ...
Chromatin immunoprecipitation for determining the association of proteins with specific genomic sequences in vivo [0.03%]
用于确定体内蛋白质与特定基因组序列关联的染色质免疫沉淀法
Oscar Aparicio,Joseph V Geisberg,Kevin Struhl
Oscar Aparicio
Chromatin immunoprecipitation (ChIP) is a powerful and widely applied technique for detecting the association of individual proteins with specific genomic regions in vivo. Live cells are treated with formaldehyde to generate protein-protein...
Peter Schuck,Lisa F Boyd,Peter S Andersen
Peter Schuck
In recent years, optical evanescent wave biosensors have been used to characterize protein-protein interactions, including determination of equilibrium binding constants and bimolecular rate constants. This surface binding technique can pro...
Protein-protein interactions identified by pull-down experiments and mass spectrometry [0.03%]
基于拉下实验和质谱技术鉴定的蛋白质-蛋白质相互作用
Adam Brymora,Valentina A Valova,Phillip J Robinson
Adam Brymora
The aim of this unit is to provide a method for the identification of new protein-protein interactions. Pull-down experiments with GST fusion proteins attached to glutathione beads are a screening technique for identification of protein-pro...
Chung-Wai Chow,Gregory P Downey,Sergio Grinstein
Chung-Wai Chow
Phagocytosis is an essential component of the innate immune response. Invading microorganisms are engulfed by neutrophils and macrophages, and subsequently killed by a complex series of reactions that require fusion of the phagocytic vacuol...
Roumen Pankov
Roumen Pankov
This unit provides a basic protocol for assaying the level of activity, as well as the activation capacity and dynamics of inhibition of Akt/PKB in cultured cells. All these data can be obtained in a single nonradioactive experiment by stan...
Christophe Le Clainche,Marie-France Carlier
Christophe Le Clainche
Actin-based movement can be reconstituted by using microspheres functionalized with the enzymes N-WASP or ActA, which use the Arp2/3 complex and actin to catalyze the formation of a branched actin filament network that is maintained in rapi...