Andrew Osborne,Alexander Flett,Elizabeth Smythe
Andrew Osborne
Clathrin-coated pits and vesicles represent the major ports of entry into most eukaryotic cells. As well as performing housekeeping functions (e.g., allowing cells to take up essential nutrients), the endocytic pathway participates in a num...
Fluorescence lifetime imaging microscopy [0.03%]
荧光寿命成像 microscopy fluorescence lifetime imaging microscopy (flim)
Alessandro Esposito,Fred S Wouters
Alessandro Esposito
Fluorescent lifetime imaging microscopy is a powerful tool to enhance the contrast in images of biological samples and to investigate the local environment of a fluorochrome. FLIM allows the detection of protein-protein interactions and the...
Branch Craige,Gloria Salazar,Victor Faundez
Branch Craige
Synaptic vesicles are the most abundant secretory organelle in eukaryotic neural cells. Synaptic vesicles are physically distinct from other membrane-bound organelles because they are small, spherical, and highly uniform in size with a diam...
Jane Bayani,Jeremy Squire
Jane Bayani
Traditional FISH analysis has employed, at most, two colors of detection, a red-fluorescing fluorochrome and a green-fluorescing fluorochrome. The improvements in fluorescent imaging and development of heartier fluorochromes/dyes have enabl...
Jane Bayani,Jeremy A Squire
Jane Bayani
Fluorescence in situ Hybridization (FISH) involves the preparation of two main components: the DNA probe and the target DNA to which the probe will be hybridized. The DNA probe typically comes from cloned sources such as plasmids, cosmids, ...
Jane Bayani,Jeremy A Squire
Jane Bayani
Traditional banding of metaphase chromosomes allows identification of individual chromosomes and detection of gross chromosomal anomalies and abnormal chromosome structures. Giemsa (G) banding is the older and more familiar nonfluorescent t...
Jane Bayani,Jeremy A Squire
Jane Bayani
Cytogenetic specimens are prepared from short-term primary cultures established from tissue samples or from established cell lines. Cultures that are (80% confluent are treated with Colcemid to enrich for cells in mitosis. Cells are then sw...
Jane Bayani,Jeremy A Squire
Jane Bayani
This unit provides a brief overview of cytogenetic analysis of interphase ansd metaphase chromosomes. It includes a description of the methods provided in the units that follow.
Graham A Dunn,Mark R Holt,Daniel Y H Soong et al.
Graham A Dunn et al.
Fluorescence localization after photobleaching is a new method for localized photolabeling and subsequent tracking of specific molecules within living cells. The molecular species to be located carries two different fluorophores that can be...
Susan S Yamada
Susan S Yamada
Cultured keratinocytes have been used by a number of investigators in studies investigating wound repair and carcinogenesis, and they have also proven useful as a model for differentiation. This unit describes a protocol for establishing hu...