Jane Bayani,Jeremy A Squire
Jane Bayani
Mitotic figures have provided important information on the mechanisms behind observed chromosomal aberrations. Variations in cell culturing and fixation methods enhance the visualization of mitotic figures. Furthermore, immunohistochemistry...
Sister chromatid exchange [0.03%]
姐妹染色单体交换
Jane Bayani,Jeremy A Squire
Jane Bayani
Sister chromatid exchange (SCE) refers to the interchange of DNA between replication products. The technique for detecting such exchanges takes advantage of the semiconservative nature of DNA synthesis. 5'-bromodeoxyuridine (BrdU) is incorp...
Jane Bayani,Jeremy A Squire
Jane Bayani
Comparative genomic hybridization (CGH) is a screening method based on fluorescence in situ hybridization (FISH). In contrast to conventional FISH, the metaphase target is derived from a normal peripheral blood lymphocyte culture. This targ...
Erik Snapp
Erik Snapp
This unit describes strategies for designing functional fluorescent fusion protein constructs. Such constructs can be exploited as probes of cellular environments, protein dynamics, protein life histories, protein binding partners, and mark...
Inducible gene expression using an autoregulatory, tetracycline-controlled system [0.03%]
利用自调控四环素控制系统诱导基因表达
Penny Shockett,David Schatz
Penny Shockett
The protocols in this unit describe the transfection of adherent cells and the testing of resultant clones for inducible transactivator or target gene protein expression. Stably transfected fibroblast cell lines expressing transactivator an...
Analysis of cellular senescence in culture in vivo: the senescence-associated beta-galactosidase assay [0.03%]
细胞培养和活体中分析衰老的β-半乳糖苷酶试验
Debdutta Bandyopadhyay,Catherine Gatza,Lawrence A Donehower et al.
Debdutta Bandyopadhyay et al.
Replicative senescence, a process first described almost 40 years ago, entails irreversible growth arrest with sustained metabolic functions, and it is also associated with increased resistance to apoptotic signals. Interest in this process...
Beat E Schaub,Prashant Nair,Jack Rohrer
Beat E Schaub
Lysosomes are terminal degradative organelles that are found in all higher eukaryotic cells. The biogenesis of lysosomes involves the transport of various acid hydrolases and transmembrane glycoproteins from their site of synthesis in the e...
Yoav Shaul,Rony Seger
Yoav Shaul
Mitogen-activated protein kinase (MAPK) cascades are central pathways that participate in the intracellular transmission of extracellular signals. Each of the MAPK signaling cascades seems to consist of three to five tiers of protein kinase...
Maud Hertzog,Marie-France Carlier
Maud Hertzog
A very large, ever-increasing repertoire of actin-binding proteins regulates the assembly dynamics and the spatial organization of actin filaments, thus orchestrating the motile behavior of the cell. The authors describe a series of biochem...
In vivo marking of single cells in chick embryos using photoactivation of GFP [0.03%]
利用光活化GFP在鸡胚体内标记单个细胞
D A Stark,P M Kulesa
D A Stark
Selective marking of a single cell within a living embryo is often difficult due to the inaccuracy and invasiveness of standard techniques. This unit describes a minimally invasive optical protocol that uses 405-nm laser light to photoactiv...